Limits...
Life at the mesoscale: the self-organised cytoplasm and nucleoplasm.

Sear RP, Pagonabarraga I, Flaus A - BMC Biophys (2015)

Bottom Line: The challenges of mesoscale self-organisation were discussed at a CECAM workshop in July 2014.Biologists need approaches to observe highly dynamic, low affinity, low specificity associations and to perturb single structures, while biological physicists and biomathematicians need to work closely with biologists to build and validate quantitative models.A table of terminology is included to facilitate multidisciplinary efforts to reveal the richness and diversity of mesoscale cell biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, University of Surrey, GU2 7XH Guildford, Surrey UK.

ABSTRACT
The cell contains highly dynamic structures exploiting physical principles of self-organisation at the mesoscale (100 nm to 10 μm). Examples include non-membrane bound cytoplasmic bodies, cytoskeleton-based motor networks and multi-scale chromatin organisation. The challenges of mesoscale self-organisation were discussed at a CECAM workshop in July 2014. Biologists need approaches to observe highly dynamic, low affinity, low specificity associations and to perturb single structures, while biological physicists and biomathematicians need to work closely with biologists to build and validate quantitative models. A table of terminology is included to facilitate multidisciplinary efforts to reveal the richness and diversity of mesoscale cell biology.

No MeSH data available.


Mesoscale lengths in context. The self-organised structures considered in the workshop are mesoscale structures. We define mesoscales as length-scales larger than discrete molecular complexes yet remaining intracellular. These limits are bounded below by ribosomal diameters (25 nm) as the molecular scale, and above by typical model cell diameters. For E. coli, this is approximately 1 μm, while for human cells it is of order 10 μm. Unusually large cell diameters such as neurons (100 μm) and amphibian oocytes (1 mm) mean the upper limit will depend on the cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4374369&req=5

Fig1: Mesoscale lengths in context. The self-organised structures considered in the workshop are mesoscale structures. We define mesoscales as length-scales larger than discrete molecular complexes yet remaining intracellular. These limits are bounded below by ribosomal diameters (25 nm) as the molecular scale, and above by typical model cell diameters. For E. coli, this is approximately 1 μm, while for human cells it is of order 10 μm. Unusually large cell diameters such as neurons (100 μm) and amphibian oocytes (1 mm) mean the upper limit will depend on the cell.

Mentions: Here we define the mesoscale as being length scales larger than individual molecular machines such as ribosomes, but no larger than the size of the cell. In Figure 1 we place this range of length scales in context. With new experimental techniques we can see that on these length scales the cytoplasm and nucleoplasm are neither uniform nor static, but are highly organised and often highly dynamic. We have been dramatically underestimating the extent of this mesoscale self-organisation, and its role in key processes of the cell function. As our understanding increases, the number of mesoscale structures we are aware of is growing. We have illustrated a selection of the structures discussed at the workshop in Figure 2. This figure illustrates “mesoscale cell biology” in the sense that it is organisation of the cell interior on mid-range length scales.Figure 1


Life at the mesoscale: the self-organised cytoplasm and nucleoplasm.

Sear RP, Pagonabarraga I, Flaus A - BMC Biophys (2015)

Mesoscale lengths in context. The self-organised structures considered in the workshop are mesoscale structures. We define mesoscales as length-scales larger than discrete molecular complexes yet remaining intracellular. These limits are bounded below by ribosomal diameters (25 nm) as the molecular scale, and above by typical model cell diameters. For E. coli, this is approximately 1 μm, while for human cells it is of order 10 μm. Unusually large cell diameters such as neurons (100 μm) and amphibian oocytes (1 mm) mean the upper limit will depend on the cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4374369&req=5

Fig1: Mesoscale lengths in context. The self-organised structures considered in the workshop are mesoscale structures. We define mesoscales as length-scales larger than discrete molecular complexes yet remaining intracellular. These limits are bounded below by ribosomal diameters (25 nm) as the molecular scale, and above by typical model cell diameters. For E. coli, this is approximately 1 μm, while for human cells it is of order 10 μm. Unusually large cell diameters such as neurons (100 μm) and amphibian oocytes (1 mm) mean the upper limit will depend on the cell.
Mentions: Here we define the mesoscale as being length scales larger than individual molecular machines such as ribosomes, but no larger than the size of the cell. In Figure 1 we place this range of length scales in context. With new experimental techniques we can see that on these length scales the cytoplasm and nucleoplasm are neither uniform nor static, but are highly organised and often highly dynamic. We have been dramatically underestimating the extent of this mesoscale self-organisation, and its role in key processes of the cell function. As our understanding increases, the number of mesoscale structures we are aware of is growing. We have illustrated a selection of the structures discussed at the workshop in Figure 2. This figure illustrates “mesoscale cell biology” in the sense that it is organisation of the cell interior on mid-range length scales.Figure 1

Bottom Line: The challenges of mesoscale self-organisation were discussed at a CECAM workshop in July 2014.Biologists need approaches to observe highly dynamic, low affinity, low specificity associations and to perturb single structures, while biological physicists and biomathematicians need to work closely with biologists to build and validate quantitative models.A table of terminology is included to facilitate multidisciplinary efforts to reveal the richness and diversity of mesoscale cell biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, University of Surrey, GU2 7XH Guildford, Surrey UK.

ABSTRACT
The cell contains highly dynamic structures exploiting physical principles of self-organisation at the mesoscale (100 nm to 10 μm). Examples include non-membrane bound cytoplasmic bodies, cytoskeleton-based motor networks and multi-scale chromatin organisation. The challenges of mesoscale self-organisation were discussed at a CECAM workshop in July 2014. Biologists need approaches to observe highly dynamic, low affinity, low specificity associations and to perturb single structures, while biological physicists and biomathematicians need to work closely with biologists to build and validate quantitative models. A table of terminology is included to facilitate multidisciplinary efforts to reveal the richness and diversity of mesoscale cell biology.

No MeSH data available.