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Isolation of Leptospira interrogans serovar Hardjoprajitno from a calf with clinical leptospirosis in Chile.

Salgado M, Otto B, Moroni M, Sandoval E, Reinhardt G, Boqvist S, Encina C, Muñoz-Zanzi C - BMC Vet. Res. (2015)

Bottom Line: A urine sample was submitted to conventional bacteriological analysis together with highly specific molecular biology typing tools, in order to unravel the specific Leptospira specie and serovar associated with this clinical case.Leptospira interrogans serovar Hardjoprajitno was associated with the investigated calf clinical case.This information adds to the value of serologic results commonly reported, which encourage vaccination improvements to match circulating strains.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Edificio Instapanel, Campus Isla Teja, CC 567, Valdivia, Chile. miguelsalgado@uach.cl.

ABSTRACT

Background: Although Leptospira isolation has been reported in Chilean cattle, only serological evidence of serovar Hardjo bovis infection has been routinely reported. The present report provides characterization of the pathological presentation and etiology of a clinical case of leptospirosis in a calf from the Los Rios Region in Chile.

Case presentation: In a dairy herd in southern Chile, 11 of 130 calves died after presenting signs such as depression and red-tinged urine. One of these calves, a female of eight months, was necropsied, and all the pathological findings were consistent with Leptospira infection. A urine sample was submitted to conventional bacteriological analysis together with highly specific molecular biology typing tools, in order to unravel the specific Leptospira specie and serovar associated with this clinical case. A significant finding of this study was that the obtained isolate was confirmed by PCR as L. interrogans, its VNTR profile properly matching with L. interrogans Hardjoprajitno as well as its specific genomic identity revealed by secY gen.

Conclusion: Leptospira interrogans serovar Hardjoprajitno was associated with the investigated calf clinical case. This information adds to the value of serologic results commonly reported, which encourage vaccination improvements to match circulating strains. In addition, this finding represents the first case report of this serovar in Chilean cattle.

No MeSH data available.


Related in: MedlinePlus

ClustalW aligment for 202 bp fragment,SecYgen.
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Fig3: ClustalW aligment for 202 bp fragment,SecYgen.

Mentions: The isolate obtained in this study was confirmed by PCR as L. interrogans and its VNTR profile properly matched with L. interrogans type Hardjo prajitno (Figure 2). The secY gene alignment done by clustalW did reveal sequence identity strain belonging to the species L. interrogans serovar Hardjo prajitno (Figure 3). This confirms that L. interrogans type Hardjo prajitno is associated with acute infection of cattle in Chile. Previous studies have shown that the abortion rate after Leptospira borgpetersenii serovar Hardjo bovis infection is 3 to 10% whereas the rate increases up to 30% for L. Hardjo prajitno infection [3-7], which underscores the importance of this serovar. The clinical information in the presented study was conveniently complemented with bacteriological findings to describe the isolated strain affecting the clinical case presented.Figure 2


Isolation of Leptospira interrogans serovar Hardjoprajitno from a calf with clinical leptospirosis in Chile.

Salgado M, Otto B, Moroni M, Sandoval E, Reinhardt G, Boqvist S, Encina C, Muñoz-Zanzi C - BMC Vet. Res. (2015)

ClustalW aligment for 202 bp fragment,SecYgen.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4374366&req=5

Fig3: ClustalW aligment for 202 bp fragment,SecYgen.
Mentions: The isolate obtained in this study was confirmed by PCR as L. interrogans and its VNTR profile properly matched with L. interrogans type Hardjo prajitno (Figure 2). The secY gene alignment done by clustalW did reveal sequence identity strain belonging to the species L. interrogans serovar Hardjo prajitno (Figure 3). This confirms that L. interrogans type Hardjo prajitno is associated with acute infection of cattle in Chile. Previous studies have shown that the abortion rate after Leptospira borgpetersenii serovar Hardjo bovis infection is 3 to 10% whereas the rate increases up to 30% for L. Hardjo prajitno infection [3-7], which underscores the importance of this serovar. The clinical information in the presented study was conveniently complemented with bacteriological findings to describe the isolated strain affecting the clinical case presented.Figure 2

Bottom Line: A urine sample was submitted to conventional bacteriological analysis together with highly specific molecular biology typing tools, in order to unravel the specific Leptospira specie and serovar associated with this clinical case.Leptospira interrogans serovar Hardjoprajitno was associated with the investigated calf clinical case.This information adds to the value of serologic results commonly reported, which encourage vaccination improvements to match circulating strains.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Edificio Instapanel, Campus Isla Teja, CC 567, Valdivia, Chile. miguelsalgado@uach.cl.

ABSTRACT

Background: Although Leptospira isolation has been reported in Chilean cattle, only serological evidence of serovar Hardjo bovis infection has been routinely reported. The present report provides characterization of the pathological presentation and etiology of a clinical case of leptospirosis in a calf from the Los Rios Region in Chile.

Case presentation: In a dairy herd in southern Chile, 11 of 130 calves died after presenting signs such as depression and red-tinged urine. One of these calves, a female of eight months, was necropsied, and all the pathological findings were consistent with Leptospira infection. A urine sample was submitted to conventional bacteriological analysis together with highly specific molecular biology typing tools, in order to unravel the specific Leptospira specie and serovar associated with this clinical case. A significant finding of this study was that the obtained isolate was confirmed by PCR as L. interrogans, its VNTR profile properly matching with L. interrogans Hardjoprajitno as well as its specific genomic identity revealed by secY gen.

Conclusion: Leptospira interrogans serovar Hardjoprajitno was associated with the investigated calf clinical case. This information adds to the value of serologic results commonly reported, which encourage vaccination improvements to match circulating strains. In addition, this finding represents the first case report of this serovar in Chilean cattle.

No MeSH data available.


Related in: MedlinePlus