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Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender.

Pandey P, Reddy NV, Rao VA, Saxena A, Chaudhary CP - Contemp Clin Dent (2015)

Bottom Line: Salivary antioxidant activity is measured with spectrophotometer by an adaptation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonate) assays.Salivary total protein and total antioxidant level were proved to be statistically significant for male children only.Calcium content of saliva was found to be more in caries-free group and increased with age.

View Article: PubMed Central - PubMed

Affiliation: Department of Pedodontics and Preventive Dentistry, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Purpose: The aim of the study was to evaluate salivary flow rate, pH, buffering capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries, age and gender.

Materials and methods: The study population consisted of 120 healthy children aged 7-15 years that was further divided into two groups: 7-10 years and 11-15 years. In this 60 children with DMFS/dfs = 0 and 60 children with DMFS/dfs ≥5 were included. The subjects were divided into two groups; Group A: Children with DMFS/dfs = 0 (caries-free) Group B: Children with DMFS/dfs ≥5 (caries active). Unstimulated saliva samples were collected from all groups. Flow rates were determined, and samples analyzed for pH, buffer capacity, calcium, total protein and total antioxidant status. Salivary antioxidant activity is measured with spectrophotometer by an adaptation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonate) assays.

Results: The mean difference of the two groups; caries-free and caries active were proved to be statistically significant (P < 0.05) for salivary calcium, total protein and total antioxidant level for both the sexes in the age group 7-10 years and for the age 11-15 years the mean difference of the two groups were proved to be statistically significant (P < 0.05) for salivary calcium level for both the sexes. Salivary total protein and total antioxidant level were proved to be statistically significant for male children only.

Conclusions: In general, total protein and total antioxidants in saliva were increased with caries activity. Calcium content of saliva was found to be more in caries-free group and increased with age.

No MeSH data available.


Related in: MedlinePlus

Measuring calcium
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Figure 3: Measuring calcium

Mentions: The total protein and calcium levels of the samples are measured by autoanalyzer [Figure 3]. Measurement of protein content is based on biuret method [Figure 4], and alkaline copper reagent is used. The protein in saliva produces an alkaline copper-protein chelate when combined with the reagent. The resulting increase in absorbency is monitored by a detector at 545 nm. The observed rate of chelate formation is directly proportional to the total protein concentration in the sample.


Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender.

Pandey P, Reddy NV, Rao VA, Saxena A, Chaudhary CP - Contemp Clin Dent (2015)

Measuring calcium
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374323&req=5

Figure 3: Measuring calcium
Mentions: The total protein and calcium levels of the samples are measured by autoanalyzer [Figure 3]. Measurement of protein content is based on biuret method [Figure 4], and alkaline copper reagent is used. The protein in saliva produces an alkaline copper-protein chelate when combined with the reagent. The resulting increase in absorbency is monitored by a detector at 545 nm. The observed rate of chelate formation is directly proportional to the total protein concentration in the sample.

Bottom Line: Salivary antioxidant activity is measured with spectrophotometer by an adaptation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonate) assays.Salivary total protein and total antioxidant level were proved to be statistically significant for male children only.Calcium content of saliva was found to be more in caries-free group and increased with age.

View Article: PubMed Central - PubMed

Affiliation: Department of Pedodontics and Preventive Dentistry, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Purpose: The aim of the study was to evaluate salivary flow rate, pH, buffering capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries, age and gender.

Materials and methods: The study population consisted of 120 healthy children aged 7-15 years that was further divided into two groups: 7-10 years and 11-15 years. In this 60 children with DMFS/dfs = 0 and 60 children with DMFS/dfs ≥5 were included. The subjects were divided into two groups; Group A: Children with DMFS/dfs = 0 (caries-free) Group B: Children with DMFS/dfs ≥5 (caries active). Unstimulated saliva samples were collected from all groups. Flow rates were determined, and samples analyzed for pH, buffer capacity, calcium, total protein and total antioxidant status. Salivary antioxidant activity is measured with spectrophotometer by an adaptation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonate) assays.

Results: The mean difference of the two groups; caries-free and caries active were proved to be statistically significant (P < 0.05) for salivary calcium, total protein and total antioxidant level for both the sexes in the age group 7-10 years and for the age 11-15 years the mean difference of the two groups were proved to be statistically significant (P < 0.05) for salivary calcium level for both the sexes. Salivary total protein and total antioxidant level were proved to be statistically significant for male children only.

Conclusions: In general, total protein and total antioxidants in saliva were increased with caries activity. Calcium content of saliva was found to be more in caries-free group and increased with age.

No MeSH data available.


Related in: MedlinePlus