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Prevalence of glutathione S-transferase M1 polymorphism in tobacco users, oral leukoplakia and oral squamous cell carcinoma patients in South Indian population: A polymerase chain reaction study.

Tanwar R, Iyengar AR, Nagesh KS, Patil S, Subhash BV - Contemp Clin Dent (2015)

Bottom Line: Factors that influence tobacco-exposed individuals developing a malignancy may include a combination of total tobacco exposure and genetic susceptibility.This case-control study was conducted in hospital setting on South Indian population.Three milliliter of blood was collected and transported under cold cycle and taken for evaluation of GSTM1 polymorphism using Multiplex Polymerase Chain Reaction.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Medicine and Radiology, SGT Dental College, Haryana, India.

ABSTRACT

Context: Tobacco abuse is a well-known risk factor for potentially malignant disorders as well as oral squamous cell carcinoma (SCC). Factors that influence tobacco-exposed individuals developing a malignancy may include a combination of total tobacco exposure and genetic susceptibility.

Aim: This study was undertaken to determine the prevalence of the glutathione S-transferase M1 (GSTM1) polymorphism in oral leukoplakia and oral SCC patients in South Indian population.

Settings and design: This case-control study was conducted in hospital setting on South Indian population.

Materials and methods: Totally, 280 subjects with a history of tobacco use, oral leukoplakia, oral SCC were included in this study. Three milliliter of blood was collected and transported under cold cycle and taken for evaluation of GSTM1 polymorphism using Multiplex Polymerase Chain Reaction.

Results and discussion: On comparing the prevalence of GSTM1 polymorphism among the group with subjects with habits and no oral lesions, oral leukoplakia and oral SCC, it was observed that there was a statistically significant association between GSTM1 polymorphism and the different groups (P < 0.01).

Conclusion: The lack of GSTM1 activity would make the oral tissues more susceptible to action of tobacco carcinogens and to the development of a high-grade level of dysplasia in oral leukoplakia and thereby increases the susceptibility of lesion to undergo malignant changes.

No MeSH data available.


Related in: MedlinePlus

Gel electrophoresis of Multiplex Polymerase Chain Reaction amplified products showing individuals for glutathione S-transferase M1 (GSTM1) polymorphism. Blue arrowhead indicating single band suggestive of GSTM1  polymorphism and red arrowhead indicating double band suggestive of GSTM1 polymorphism present
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Figure 1: Gel electrophoresis of Multiplex Polymerase Chain Reaction amplified products showing individuals for glutathione S-transferase M1 (GSTM1) polymorphism. Blue arrowhead indicating single band suggestive of GSTM1 polymorphism and red arrowhead indicating double band suggestive of GSTM1 polymorphism present

Mentions: The albumin band was present in all samples since it was the internal positive control that indicates DNA was present in the sample. Homozygote (+) samples will show double band for GSTM1 of the size 273 bp fragment. The homozygote samples will be missing the band for the gene that is deleted (one or both bands). In this PCR assay, the absence of a 273-bp fragment indicated the GSTM1 genotype which was denoted by presence of single band for the gene GSTM1 instead of two bands for the gene GSTM1 [Figure 1].


Prevalence of glutathione S-transferase M1 polymorphism in tobacco users, oral leukoplakia and oral squamous cell carcinoma patients in South Indian population: A polymerase chain reaction study.

Tanwar R, Iyengar AR, Nagesh KS, Patil S, Subhash BV - Contemp Clin Dent (2015)

Gel electrophoresis of Multiplex Polymerase Chain Reaction amplified products showing individuals for glutathione S-transferase M1 (GSTM1) polymorphism. Blue arrowhead indicating single band suggestive of GSTM1  polymorphism and red arrowhead indicating double band suggestive of GSTM1 polymorphism present
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374322&req=5

Figure 1: Gel electrophoresis of Multiplex Polymerase Chain Reaction amplified products showing individuals for glutathione S-transferase M1 (GSTM1) polymorphism. Blue arrowhead indicating single band suggestive of GSTM1 polymorphism and red arrowhead indicating double band suggestive of GSTM1 polymorphism present
Mentions: The albumin band was present in all samples since it was the internal positive control that indicates DNA was present in the sample. Homozygote (+) samples will show double band for GSTM1 of the size 273 bp fragment. The homozygote samples will be missing the band for the gene that is deleted (one or both bands). In this PCR assay, the absence of a 273-bp fragment indicated the GSTM1 genotype which was denoted by presence of single band for the gene GSTM1 instead of two bands for the gene GSTM1 [Figure 1].

Bottom Line: Factors that influence tobacco-exposed individuals developing a malignancy may include a combination of total tobacco exposure and genetic susceptibility.This case-control study was conducted in hospital setting on South Indian population.Three milliliter of blood was collected and transported under cold cycle and taken for evaluation of GSTM1 polymorphism using Multiplex Polymerase Chain Reaction.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Medicine and Radiology, SGT Dental College, Haryana, India.

ABSTRACT

Context: Tobacco abuse is a well-known risk factor for potentially malignant disorders as well as oral squamous cell carcinoma (SCC). Factors that influence tobacco-exposed individuals developing a malignancy may include a combination of total tobacco exposure and genetic susceptibility.

Aim: This study was undertaken to determine the prevalence of the glutathione S-transferase M1 (GSTM1) polymorphism in oral leukoplakia and oral SCC patients in South Indian population.

Settings and design: This case-control study was conducted in hospital setting on South Indian population.

Materials and methods: Totally, 280 subjects with a history of tobacco use, oral leukoplakia, oral SCC were included in this study. Three milliliter of blood was collected and transported under cold cycle and taken for evaluation of GSTM1 polymorphism using Multiplex Polymerase Chain Reaction.

Results and discussion: On comparing the prevalence of GSTM1 polymorphism among the group with subjects with habits and no oral lesions, oral leukoplakia and oral SCC, it was observed that there was a statistically significant association between GSTM1 polymorphism and the different groups (P < 0.01).

Conclusion: The lack of GSTM1 activity would make the oral tissues more susceptible to action of tobacco carcinogens and to the development of a high-grade level of dysplasia in oral leukoplakia and thereby increases the susceptibility of lesion to undergo malignant changes.

No MeSH data available.


Related in: MedlinePlus