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Pomegranate extract attenuates unilateral ureteral obstruction-induced renal damage by reducing oxidative stress.

Otunctemur A, Ozbek E, Cakir SS, Polat EC, Dursun M, Cekmen M, Somay A, Ozbay N - Urol Ann (2015 Apr-Jun)

Bottom Line: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.There was no difference significantly for urea-creatinine levels between groups.Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Okmeydani Training and Research Hospital, Istanbul, Turkey.

ABSTRACT

Aims: Ureteral obstruction may cause permanent kidney damage at late period. We know that the pomegranate extract (PE) play a strong role on removal of free oxygen radicals and prevention of oxidative stress. In the current study study, we evaluated the effect of PE on kidney damage after unilateral ureteral obstruction (UUO).

Settings and design: A total of 32 rats were divided into four groups. Group 1 was a control, Group 2 was a sham, Group 3 was rats with UUO and Group 4 was rats with UUO that were given PE (oral 100 μL/day). After 14 days, rats were killed and their kidneys were taken and blood analysis was performed.

Subjects and methods: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.

Statistical analysis used: Statistical analyses were performed by the Chi-square test and one-way analysis of variance.

Results: There was no difference significantly for urea-creatinine levels between groups. Pathologically, there was serious tubular necrosis, mononuclear cell infiltration and fibrosis in Group 3, and there was significantly decreasing for tubular necrosis, mononuclear cell infiltration and fibrosis in Group 4 (P < 0.005). Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

Conclusions: We think that the PE prevents kidney damage by decreasing oxidative stress in kidney.

No MeSH data available.


Related in: MedlinePlus

(a) Normal kidney morphology in a sham group. (b) Leukocyte infiltration was observed in the peritubular interstitium of the unilateral ureteral obstruction. (c) Leukocyte infiltration was reduced in the PE-treated group (H and E, ×400)
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Figure 2: (a) Normal kidney morphology in a sham group. (b) Leukocyte infiltration was observed in the peritubular interstitium of the unilateral ureteral obstruction. (c) Leukocyte infiltration was reduced in the PE-treated group (H and E, ×400)

Mentions: Histopathologic examination was normal in rats with only sham operation (Group 2) and in rats with no operation (Group 1). Severe leukocyte infiltration was observed in the periglomerular and peritubular interstitium of the kidneys of the rats in Group 3 with UUO [Figure 2a and b]. Quantitative analysis of the focal leukocyte infiltration area in the interstitium showed that leukocyte infiltration was significantly reduced in rats that received UUO + PE (Group 4) [Figure 2c]. UUO caused a significant interstitial fibrosis in rats that received no treatment (Group 3), and the percentage area of interstitial fibrosis in the kidney of rats with UUO that received no treatment was significantly greater than that of rats with UUO that received PE (Group 4) [Figure 3a–c]. These changes are summarized in Table 3.


Pomegranate extract attenuates unilateral ureteral obstruction-induced renal damage by reducing oxidative stress.

Otunctemur A, Ozbek E, Cakir SS, Polat EC, Dursun M, Cekmen M, Somay A, Ozbay N - Urol Ann (2015 Apr-Jun)

(a) Normal kidney morphology in a sham group. (b) Leukocyte infiltration was observed in the peritubular interstitium of the unilateral ureteral obstruction. (c) Leukocyte infiltration was reduced in the PE-treated group (H and E, ×400)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374253&req=5

Figure 2: (a) Normal kidney morphology in a sham group. (b) Leukocyte infiltration was observed in the peritubular interstitium of the unilateral ureteral obstruction. (c) Leukocyte infiltration was reduced in the PE-treated group (H and E, ×400)
Mentions: Histopathologic examination was normal in rats with only sham operation (Group 2) and in rats with no operation (Group 1). Severe leukocyte infiltration was observed in the periglomerular and peritubular interstitium of the kidneys of the rats in Group 3 with UUO [Figure 2a and b]. Quantitative analysis of the focal leukocyte infiltration area in the interstitium showed that leukocyte infiltration was significantly reduced in rats that received UUO + PE (Group 4) [Figure 2c]. UUO caused a significant interstitial fibrosis in rats that received no treatment (Group 3), and the percentage area of interstitial fibrosis in the kidney of rats with UUO that received no treatment was significantly greater than that of rats with UUO that received PE (Group 4) [Figure 3a–c]. These changes are summarized in Table 3.

Bottom Line: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.There was no difference significantly for urea-creatinine levels between groups.Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Okmeydani Training and Research Hospital, Istanbul, Turkey.

ABSTRACT

Aims: Ureteral obstruction may cause permanent kidney damage at late period. We know that the pomegranate extract (PE) play a strong role on removal of free oxygen radicals and prevention of oxidative stress. In the current study study, we evaluated the effect of PE on kidney damage after unilateral ureteral obstruction (UUO).

Settings and design: A total of 32 rats were divided into four groups. Group 1 was a control, Group 2 was a sham, Group 3 was rats with UUO and Group 4 was rats with UUO that were given PE (oral 100 μL/day). After 14 days, rats were killed and their kidneys were taken and blood analysis was performed.

Subjects and methods: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.

Statistical analysis used: Statistical analyses were performed by the Chi-square test and one-way analysis of variance.

Results: There was no difference significantly for urea-creatinine levels between groups. Pathologically, there was serious tubular necrosis, mononuclear cell infiltration and fibrosis in Group 3, and there was significantly decreasing for tubular necrosis, mononuclear cell infiltration and fibrosis in Group 4 (P < 0.005). Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

Conclusions: We think that the PE prevents kidney damage by decreasing oxidative stress in kidney.

No MeSH data available.


Related in: MedlinePlus