Limits...
Pomegranate extract attenuates unilateral ureteral obstruction-induced renal damage by reducing oxidative stress.

Otunctemur A, Ozbek E, Cakir SS, Polat EC, Dursun M, Cekmen M, Somay A, Ozbay N - Urol Ann (2015 Apr-Jun)

Bottom Line: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.There was no difference significantly for urea-creatinine levels between groups.Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Okmeydani Training and Research Hospital, Istanbul, Turkey.

ABSTRACT

Aims: Ureteral obstruction may cause permanent kidney damage at late period. We know that the pomegranate extract (PE) play a strong role on removal of free oxygen radicals and prevention of oxidative stress. In the current study study, we evaluated the effect of PE on kidney damage after unilateral ureteral obstruction (UUO).

Settings and design: A total of 32 rats were divided into four groups. Group 1 was a control, Group 2 was a sham, Group 3 was rats with UUO and Group 4 was rats with UUO that were given PE (oral 100 μL/day). After 14 days, rats were killed and their kidneys were taken and blood analysis was performed.

Subjects and methods: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.

Statistical analysis used: Statistical analyses were performed by the Chi-square test and one-way analysis of variance.

Results: There was no difference significantly for urea-creatinine levels between groups. Pathologically, there was serious tubular necrosis, mononuclear cell infiltration and fibrosis in Group 3, and there was significantly decreasing for tubular necrosis, mononuclear cell infiltration and fibrosis in Group 4 (P < 0.005). Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

Conclusions: We think that the PE prevents kidney damage by decreasing oxidative stress in kidney.

No MeSH data available.


Related in: MedlinePlus

(a) Normal tubulus and glomerules in kidney kortex H and E ×100 (control group). (b) Normal tubulus and glomerules in kidney kortex H and E ×100 (sham group). (c) Severe tubular total necrosis, tubular degeneration and epithelial vacuolization in the proximal tubules H and E ×400 (unilateral ureteral obstruction [UUO] group). (d) Mild epithelial vacuolization in the proximal tubules and normal glomerules H and E ×100 (UUO + PE treated group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4374253&req=5

Figure 1: (a) Normal tubulus and glomerules in kidney kortex H and E ×100 (control group). (b) Normal tubulus and glomerules in kidney kortex H and E ×100 (sham group). (c) Severe tubular total necrosis, tubular degeneration and epithelial vacuolization in the proximal tubules H and E ×400 (unilateral ureteral obstruction [UUO] group). (d) Mild epithelial vacuolization in the proximal tubules and normal glomerules H and E ×100 (UUO + PE treated group)

Mentions: Histopathological examination of kidney showed that no pathologic findings in the control group [Figure 1a]. In rats with UUO, there were mild and severe tubular necrosis in the proximal tubules compared control and sham groups [Figure 1b and c]. In rats treated with UUO + PE, despite the presence of mild tubular degeneration and tubular necrosis are less severe, and glomeruli maintained a better morphology when compared with UUO group [Figure 1d].


Pomegranate extract attenuates unilateral ureteral obstruction-induced renal damage by reducing oxidative stress.

Otunctemur A, Ozbek E, Cakir SS, Polat EC, Dursun M, Cekmen M, Somay A, Ozbay N - Urol Ann (2015 Apr-Jun)

(a) Normal tubulus and glomerules in kidney kortex H and E ×100 (control group). (b) Normal tubulus and glomerules in kidney kortex H and E ×100 (sham group). (c) Severe tubular total necrosis, tubular degeneration and epithelial vacuolization in the proximal tubules H and E ×400 (unilateral ureteral obstruction [UUO] group). (d) Mild epithelial vacuolization in the proximal tubules and normal glomerules H and E ×100 (UUO + PE treated group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374253&req=5

Figure 1: (a) Normal tubulus and glomerules in kidney kortex H and E ×100 (control group). (b) Normal tubulus and glomerules in kidney kortex H and E ×100 (sham group). (c) Severe tubular total necrosis, tubular degeneration and epithelial vacuolization in the proximal tubules H and E ×400 (unilateral ureteral obstruction [UUO] group). (d) Mild epithelial vacuolization in the proximal tubules and normal glomerules H and E ×100 (UUO + PE treated group)
Mentions: Histopathological examination of kidney showed that no pathologic findings in the control group [Figure 1a]. In rats with UUO, there were mild and severe tubular necrosis in the proximal tubules compared control and sham groups [Figure 1b and c]. In rats treated with UUO + PE, despite the presence of mild tubular degeneration and tubular necrosis are less severe, and glomeruli maintained a better morphology when compared with UUO group [Figure 1d].

Bottom Line: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.There was no difference significantly for urea-creatinine levels between groups.Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Okmeydani Training and Research Hospital, Istanbul, Turkey.

ABSTRACT

Aims: Ureteral obstruction may cause permanent kidney damage at late period. We know that the pomegranate extract (PE) play a strong role on removal of free oxygen radicals and prevention of oxidative stress. In the current study study, we evaluated the effect of PE on kidney damage after unilateral ureteral obstruction (UUO).

Settings and design: A total of 32 rats were divided into four groups. Group 1 was a control, Group 2 was a sham, Group 3 was rats with UUO and Group 4 was rats with UUO that were given PE (oral 100 μL/day). After 14 days, rats were killed and their kidneys were taken and blood analysis was performed.

Subjects and methods: Tubular necrosis, mononuclear cell infiltration, and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys.

Statistical analysis used: Statistical analyses were performed by the Chi-square test and one-way analysis of variance.

Results: There was no difference significantly for urea-creatinine levels between groups. Pathologically, there was serious tubular necrosis, mononuclear cell infiltration and fibrosis in Group 3, and there was significantly decreasing for tubular necrosis, mononuclear cell infiltration and fibrosis in Group 4 (P < 0.005). Furthermore, there was significantly increasing for NO and MDA levels; decreasing for GSH levels in Group 3 compared the other groups (P < 0.005).

Conclusions: We think that the PE prevents kidney damage by decreasing oxidative stress in kidney.

No MeSH data available.


Related in: MedlinePlus