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Virocidal activity of Egyptian scorpion venoms against hepatitis C virus.

El-Bitar AM, Sarhan MM, Aoki C, Takahara Y, Komoto M, Deng L, Moustafa MA, Hotta H - Virol. J. (2015)

Bottom Line: Development of well-tolerated regimens with high cure rates and fewer side effects is still much needed.S. maurus palmatus venom is considered as a good natural source for characterization and development of novel anti-HCV agents targeting the entry step.To our knowledge, this is the first report describing antiviral activities of Egyptian scorpion venoms against HCV, and may open a new approach towards discovering antiviral compounds derived from scorpion venoms.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt. sci.elbitar@gmail.com.

ABSTRACT

Background: Hepatitis C virus (HCV) is a major global health problem, causing chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Development of well-tolerated regimens with high cure rates and fewer side effects is still much needed. Recently, natural antimicrobial peptides (AMPs) are attracting more attention as biological compounds and can be a good template to develop therapeutic agents, including antiviral agents against a variety of viruses. Various AMPs have been characterized from the venom of different venomous animals including scorpions.

Methods: The possible antiviral activities of crude venoms obtained from five Egyptian scorpion species (Leiurus quinquestriatus, Androctonus amoreuxi, A. australis, A. bicolor and Scorpio maurus palmatus) were evaluated by a cell culture method using Huh7.5 cells and the J6/JFH1-P47 strain of HCV. Time-of-addition experiments and inactivation of enzymatic activities of the venoms were carried out to determine the characteristics of the anti-HCV activities.

Results: S. maurus palmatus and A. australis venoms showed anti-HCV activities, with 50% inhibitory concentrations (IC₅₀) being 6.3 ± 1.6 and 88.3 ± 5.8 μg/ml, respectively. S. maurus palmatus venom (30 μg/ml) impaired HCV infectivity in culture medium, but not inside the cells, through virocidal effect. The anti-HCV activity of this venom was not inhibited by a metalloprotease inhibitor or heating at 60°C. The antiviral activity was directed preferentially against HCV.

Conclusions: S. maurus palmatus venom is considered as a good natural source for characterization and development of novel anti-HCV agents targeting the entry step. To our knowledge, this is the first report describing antiviral activities of Egyptian scorpion venoms against HCV, and may open a new approach towards discovering antiviral compounds derived from scorpion venoms.

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Dose-dependent anti-HCV activity ofS. maurus palmatusvenom. A fixed amount of HCV was mixed with serial dilutions of S. maurus palmatus venom and inoculated to Huh7.5 cells at a multiplicity of infection of 0.5 pfu/cell. After virus adsorption, the cells were cultured with the same concentrations of the venom for 46 hr. The culture supernatants were harvested and titrated for virus infectivity. Percent inhibitions of HCV infectivity by the venom at the concentrations of 0.1 to 100 μg/ml are shown. Data represent means ± SEM of the data obtained from two independent experiments.
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Fig1: Dose-dependent anti-HCV activity ofS. maurus palmatusvenom. A fixed amount of HCV was mixed with serial dilutions of S. maurus palmatus venom and inoculated to Huh7.5 cells at a multiplicity of infection of 0.5 pfu/cell. After virus adsorption, the cells were cultured with the same concentrations of the venom for 46 hr. The culture supernatants were harvested and titrated for virus infectivity. Percent inhibitions of HCV infectivity by the venom at the concentrations of 0.1 to 100 μg/ml are shown. Data represent means ± SEM of the data obtained from two independent experiments.

Mentions: Anti-HCV activities of crude venoms of five Egyptian scorpion species were tested. As shown in Table 1, A. australis and S. maurus palmatus showed anti-HCV activities, with IC50 being 88.3 ± 5.8 and 6.3 ± 1.6 μg/ml, respectively. Their CC50 were >300 and >100 μg/ml, respectively, with their selectivity indexes (SI; CC50/IC50) being >3.4 and >15.8, respectively. Crude venoms of the other three scorpion species did not exhibit significant anti-HCV activities at the concentration of 100 μg/ml. Dose-dependent anti-HCV activity of S. maurus palmatus is shown in Figure 1.Table 1


Virocidal activity of Egyptian scorpion venoms against hepatitis C virus.

El-Bitar AM, Sarhan MM, Aoki C, Takahara Y, Komoto M, Deng L, Moustafa MA, Hotta H - Virol. J. (2015)

Dose-dependent anti-HCV activity ofS. maurus palmatusvenom. A fixed amount of HCV was mixed with serial dilutions of S. maurus palmatus venom and inoculated to Huh7.5 cells at a multiplicity of infection of 0.5 pfu/cell. After virus adsorption, the cells were cultured with the same concentrations of the venom for 46 hr. The culture supernatants were harvested and titrated for virus infectivity. Percent inhibitions of HCV infectivity by the venom at the concentrations of 0.1 to 100 μg/ml are shown. Data represent means ± SEM of the data obtained from two independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4374190&req=5

Fig1: Dose-dependent anti-HCV activity ofS. maurus palmatusvenom. A fixed amount of HCV was mixed with serial dilutions of S. maurus palmatus venom and inoculated to Huh7.5 cells at a multiplicity of infection of 0.5 pfu/cell. After virus adsorption, the cells were cultured with the same concentrations of the venom for 46 hr. The culture supernatants were harvested and titrated for virus infectivity. Percent inhibitions of HCV infectivity by the venom at the concentrations of 0.1 to 100 μg/ml are shown. Data represent means ± SEM of the data obtained from two independent experiments.
Mentions: Anti-HCV activities of crude venoms of five Egyptian scorpion species were tested. As shown in Table 1, A. australis and S. maurus palmatus showed anti-HCV activities, with IC50 being 88.3 ± 5.8 and 6.3 ± 1.6 μg/ml, respectively. Their CC50 were >300 and >100 μg/ml, respectively, with their selectivity indexes (SI; CC50/IC50) being >3.4 and >15.8, respectively. Crude venoms of the other three scorpion species did not exhibit significant anti-HCV activities at the concentration of 100 μg/ml. Dose-dependent anti-HCV activity of S. maurus palmatus is shown in Figure 1.Table 1

Bottom Line: Development of well-tolerated regimens with high cure rates and fewer side effects is still much needed.S. maurus palmatus venom is considered as a good natural source for characterization and development of novel anti-HCV agents targeting the entry step.To our knowledge, this is the first report describing antiviral activities of Egyptian scorpion venoms against HCV, and may open a new approach towards discovering antiviral compounds derived from scorpion venoms.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt. sci.elbitar@gmail.com.

ABSTRACT

Background: Hepatitis C virus (HCV) is a major global health problem, causing chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Development of well-tolerated regimens with high cure rates and fewer side effects is still much needed. Recently, natural antimicrobial peptides (AMPs) are attracting more attention as biological compounds and can be a good template to develop therapeutic agents, including antiviral agents against a variety of viruses. Various AMPs have been characterized from the venom of different venomous animals including scorpions.

Methods: The possible antiviral activities of crude venoms obtained from five Egyptian scorpion species (Leiurus quinquestriatus, Androctonus amoreuxi, A. australis, A. bicolor and Scorpio maurus palmatus) were evaluated by a cell culture method using Huh7.5 cells and the J6/JFH1-P47 strain of HCV. Time-of-addition experiments and inactivation of enzymatic activities of the venoms were carried out to determine the characteristics of the anti-HCV activities.

Results: S. maurus palmatus and A. australis venoms showed anti-HCV activities, with 50% inhibitory concentrations (IC₅₀) being 6.3 ± 1.6 and 88.3 ± 5.8 μg/ml, respectively. S. maurus palmatus venom (30 μg/ml) impaired HCV infectivity in culture medium, but not inside the cells, through virocidal effect. The anti-HCV activity of this venom was not inhibited by a metalloprotease inhibitor or heating at 60°C. The antiviral activity was directed preferentially against HCV.

Conclusions: S. maurus palmatus venom is considered as a good natural source for characterization and development of novel anti-HCV agents targeting the entry step. To our knowledge, this is the first report describing antiviral activities of Egyptian scorpion venoms against HCV, and may open a new approach towards discovering antiviral compounds derived from scorpion venoms.

Show MeSH
Related in: MedlinePlus