Limits...
Spread of artemisinin-resistant Plasmodium falciparum in Myanmar: a cross-sectional survey of the K13 molecular marker.

Tun KM, Imwong M, Lwin KM, Win AA, Hlaing TM, Hlaing T, Lin K, Kyaw MP, Plewes K, Faiz MA, Dhorda M, Cheah PY, Pukrittayakamee S, Ashley EA, Anderson TJ, Nair S, McDew-White M, Flegg JA, Grist EP, Guerin P, Maude RJ, Smithuis F, Dondorp AM, Day NP, Nosten F, White NJ, Woodrow CJ - Lancet Infect Dis (2015)

Bottom Line: Artemisinin resistance extends across much of Myanmar.Appropriate therapeutic regimens should be tested urgently and implemented comprehensively if spread of artemisinin resistance to other regions is to be avoided.Wellcome Trust-Mahidol University-Oxford Tropical Medicine Research Programme and the Bill & Melinda Gates Foundation.

View Article: PubMed Central - PubMed

Affiliation: Myanmar Oxford Clinical Research Unit, Yangon, Myanmar; Defence Services Medical Research Centre, Naypyitaw, Myanmar.

Show MeSH

Related in: MedlinePlus

Location of sampling sites, sample sizes, and administrative states and regions of Myanmar, and a relief map of southeast AsiaRed circles show numbers of patients in each region.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4374103&req=5

fig1: Location of sampling sites, sample sizes, and administrative states and regions of Myanmar, and a relief map of southeast AsiaRed circles show numbers of patients in each region.

Mentions: Figure 1 shows the location of sampling sites, sample sizes, and administrative states and regions of Myanmar. Of 2378 samples tested, 940 (40%) samples produced clear sequences covering aminoacids 210–726 of the K13 gene. The overall extraction–sequencing success rates were 97% for whole blood, 84% for dried blood spots, and 26% for rapid diagnostic tests. We did not screen samples with PCR before attempting to sequence K13. 11 of 12 samples sequenced from both rapid diagnostic tests and whole blood showed concordant sequences, with the remaining sample failing to produce a sequence from the diagnostic test. Only five mixed genotypes were apparent in sequencing reads.


Spread of artemisinin-resistant Plasmodium falciparum in Myanmar: a cross-sectional survey of the K13 molecular marker.

Tun KM, Imwong M, Lwin KM, Win AA, Hlaing TM, Hlaing T, Lin K, Kyaw MP, Plewes K, Faiz MA, Dhorda M, Cheah PY, Pukrittayakamee S, Ashley EA, Anderson TJ, Nair S, McDew-White M, Flegg JA, Grist EP, Guerin P, Maude RJ, Smithuis F, Dondorp AM, Day NP, Nosten F, White NJ, Woodrow CJ - Lancet Infect Dis (2015)

Location of sampling sites, sample sizes, and administrative states and regions of Myanmar, and a relief map of southeast AsiaRed circles show numbers of patients in each region.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374103&req=5

fig1: Location of sampling sites, sample sizes, and administrative states and regions of Myanmar, and a relief map of southeast AsiaRed circles show numbers of patients in each region.
Mentions: Figure 1 shows the location of sampling sites, sample sizes, and administrative states and regions of Myanmar. Of 2378 samples tested, 940 (40%) samples produced clear sequences covering aminoacids 210–726 of the K13 gene. The overall extraction–sequencing success rates were 97% for whole blood, 84% for dried blood spots, and 26% for rapid diagnostic tests. We did not screen samples with PCR before attempting to sequence K13. 11 of 12 samples sequenced from both rapid diagnostic tests and whole blood showed concordant sequences, with the remaining sample failing to produce a sequence from the diagnostic test. Only five mixed genotypes were apparent in sequencing reads.

Bottom Line: Artemisinin resistance extends across much of Myanmar.Appropriate therapeutic regimens should be tested urgently and implemented comprehensively if spread of artemisinin resistance to other regions is to be avoided.Wellcome Trust-Mahidol University-Oxford Tropical Medicine Research Programme and the Bill & Melinda Gates Foundation.

View Article: PubMed Central - PubMed

Affiliation: Myanmar Oxford Clinical Research Unit, Yangon, Myanmar; Defence Services Medical Research Centre, Naypyitaw, Myanmar.

Show MeSH
Related in: MedlinePlus