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Anticancer effect of black tea extract in human cancer cell lines.

Koňariková K, Ježovičová M, Keresteš J, Gbelcová H, Ďuračková Z, Žitňanová I - Springerplus (2015)

Bottom Line: Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h.BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations.We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.

ABSTRACT
In this study we investigated effects of natural extract from the black tea Camellia sinensis (BTE) against human colon carcinoma cell line HT-29, human breast carcinoma cell line MCF-7, human alveolar carcinoma cell line A549 and healthy cell line NIH-3T3. We identified concentration range for cytotoxic/antiproliferative effects using MTT assay and the trypan blue assay, gel electrophoresis we employed to determine the type of cell death induced by BTE and DNA damage we determined by comet assay. Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h. BTE showed cytotoxic effects against all carcinoma cell lines, however HT-29 and MCF-7 cells were more sensitive than A549. BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations. We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation. BTE induced also DNA strand breaks and oxidative damage to DNA in carcinoma cells HT-29 and MCF-7.

No MeSH data available.


Related in: MedlinePlus

Effects of BTE on the DNA damage (%) in HT-29 (a) and MCF-7 (b) cells after 24 h treatment. DNA damage was represented by DNA strand breaks (without Fpg) and as the total DNA damage (with Fpg). Each value represents the arithmetic mean ± SD of three separate experiments (n = 3): * < 0.05 when compared with controls (HT-29 or MCF-7 cells without BTE). Maximal DNA damage (100%) for 100 comets was considered the DNA damage (TD) with the maximal score (400).
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Fig2: Effects of BTE on the DNA damage (%) in HT-29 (a) and MCF-7 (b) cells after 24 h treatment. DNA damage was represented by DNA strand breaks (without Fpg) and as the total DNA damage (with Fpg). Each value represents the arithmetic mean ± SD of three separate experiments (n = 3): * < 0.05 when compared with controls (HT-29 or MCF-7 cells without BTE). Maximal DNA damage (100%) for 100 comets was considered the DNA damage (TD) with the maximal score (400).

Mentions: We examined the DNA damage induced by BTE (0.00078 - 5 μg/mL) after 24 h of incubation. Induction of DNA strand breaks (without Fpg) and total DNA damage (with Fpg) was dose independent in both cell lines (Figure 2a,b) and percentage of DNA strand breaks was approximately the same for each concentration used (up to 55% of DNA damage). Level of total DNA damage was higher (up to 60%). HT-29 cells were less sensitive to DNA damage compared with MCF-7 cells.Figure 2


Anticancer effect of black tea extract in human cancer cell lines.

Koňariková K, Ježovičová M, Keresteš J, Gbelcová H, Ďuračková Z, Žitňanová I - Springerplus (2015)

Effects of BTE on the DNA damage (%) in HT-29 (a) and MCF-7 (b) cells after 24 h treatment. DNA damage was represented by DNA strand breaks (without Fpg) and as the total DNA damage (with Fpg). Each value represents the arithmetic mean ± SD of three separate experiments (n = 3): * < 0.05 when compared with controls (HT-29 or MCF-7 cells without BTE). Maximal DNA damage (100%) for 100 comets was considered the DNA damage (TD) with the maximal score (400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374083&req=5

Fig2: Effects of BTE on the DNA damage (%) in HT-29 (a) and MCF-7 (b) cells after 24 h treatment. DNA damage was represented by DNA strand breaks (without Fpg) and as the total DNA damage (with Fpg). Each value represents the arithmetic mean ± SD of three separate experiments (n = 3): * < 0.05 when compared with controls (HT-29 or MCF-7 cells without BTE). Maximal DNA damage (100%) for 100 comets was considered the DNA damage (TD) with the maximal score (400).
Mentions: We examined the DNA damage induced by BTE (0.00078 - 5 μg/mL) after 24 h of incubation. Induction of DNA strand breaks (without Fpg) and total DNA damage (with Fpg) was dose independent in both cell lines (Figure 2a,b) and percentage of DNA strand breaks was approximately the same for each concentration used (up to 55% of DNA damage). Level of total DNA damage was higher (up to 60%). HT-29 cells were less sensitive to DNA damage compared with MCF-7 cells.Figure 2

Bottom Line: Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h.BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations.We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.

ABSTRACT
In this study we investigated effects of natural extract from the black tea Camellia sinensis (BTE) against human colon carcinoma cell line HT-29, human breast carcinoma cell line MCF-7, human alveolar carcinoma cell line A549 and healthy cell line NIH-3T3. We identified concentration range for cytotoxic/antiproliferative effects using MTT assay and the trypan blue assay, gel electrophoresis we employed to determine the type of cell death induced by BTE and DNA damage we determined by comet assay. Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h. BTE showed cytotoxic effects against all carcinoma cell lines, however HT-29 and MCF-7 cells were more sensitive than A549. BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations. We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation. BTE induced also DNA strand breaks and oxidative damage to DNA in carcinoma cells HT-29 and MCF-7.

No MeSH data available.


Related in: MedlinePlus