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Anticancer effect of black tea extract in human cancer cell lines.

Koňariková K, Ježovičová M, Keresteš J, Gbelcová H, Ďuračková Z, Žitňanová I - Springerplus (2015)

Bottom Line: Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h.BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations.We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.

ABSTRACT
In this study we investigated effects of natural extract from the black tea Camellia sinensis (BTE) against human colon carcinoma cell line HT-29, human breast carcinoma cell line MCF-7, human alveolar carcinoma cell line A549 and healthy cell line NIH-3T3. We identified concentration range for cytotoxic/antiproliferative effects using MTT assay and the trypan blue assay, gel electrophoresis we employed to determine the type of cell death induced by BTE and DNA damage we determined by comet assay. Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h. BTE showed cytotoxic effects against all carcinoma cell lines, however HT-29 and MCF-7 cells were more sensitive than A549. BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations. We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation. BTE induced also DNA strand breaks and oxidative damage to DNA in carcinoma cells HT-29 and MCF-7.

No MeSH data available.


Related in: MedlinePlus

Agarose gel electrophoresis in cells HT-29 (a, b) and MCF-7 (c, d) treated with 1–0.00625 μg/mL; 2 – 0.0125 μg/mL; 3 – 0.025 μg/mL; 4 – 5 μg/mL of BTE for 24, 48, 72, 144 and 216 h. C – control cells without BTE, PC – positive control (L1210 cells treated with 6 μmol/L cisPt).
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Fig1: Agarose gel electrophoresis in cells HT-29 (a, b) and MCF-7 (c, d) treated with 1–0.00625 μg/mL; 2 – 0.0125 μg/mL; 3 – 0.025 μg/mL; 4 – 5 μg/mL of BTE for 24, 48, 72, 144 and 216 h. C – control cells without BTE, PC – positive control (L1210 cells treated with 6 μmol/L cisPt).

Mentions: Analysis of DNA fragmentation was performed using conventional agarose gel electrophoresis to distinguish between apoptotic cell death and other types of cell death. Cells were exposed to single administration of BTE (0.00625 - 5 μg/mL) for 72 h and repetitive administration of BTE for 216 h of incubation. In cells HT-29 we observed no apoptotic cell death during single administration of BTE but we detected apoptosis during repetitive administration of BTE after 144 and 216 h of influence (Figure 1 a,b).Figure 1


Anticancer effect of black tea extract in human cancer cell lines.

Koňariková K, Ježovičová M, Keresteš J, Gbelcová H, Ďuračková Z, Žitňanová I - Springerplus (2015)

Agarose gel electrophoresis in cells HT-29 (a, b) and MCF-7 (c, d) treated with 1–0.00625 μg/mL; 2 – 0.0125 μg/mL; 3 – 0.025 μg/mL; 4 – 5 μg/mL of BTE for 24, 48, 72, 144 and 216 h. C – control cells without BTE, PC – positive control (L1210 cells treated with 6 μmol/L cisPt).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4374083&req=5

Fig1: Agarose gel electrophoresis in cells HT-29 (a, b) and MCF-7 (c, d) treated with 1–0.00625 μg/mL; 2 – 0.0125 μg/mL; 3 – 0.025 μg/mL; 4 – 5 μg/mL of BTE for 24, 48, 72, 144 and 216 h. C – control cells without BTE, PC – positive control (L1210 cells treated with 6 μmol/L cisPt).
Mentions: Analysis of DNA fragmentation was performed using conventional agarose gel electrophoresis to distinguish between apoptotic cell death and other types of cell death. Cells were exposed to single administration of BTE (0.00625 - 5 μg/mL) for 72 h and repetitive administration of BTE for 216 h of incubation. In cells HT-29 we observed no apoptotic cell death during single administration of BTE but we detected apoptosis during repetitive administration of BTE after 144 and 216 h of influence (Figure 1 a,b).Figure 1

Bottom Line: Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h.BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations.We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.

ABSTRACT
In this study we investigated effects of natural extract from the black tea Camellia sinensis (BTE) against human colon carcinoma cell line HT-29, human breast carcinoma cell line MCF-7, human alveolar carcinoma cell line A549 and healthy cell line NIH-3T3. We identified concentration range for cytotoxic/antiproliferative effects using MTT assay and the trypan blue assay, gel electrophoresis we employed to determine the type of cell death induced by BTE and DNA damage we determined by comet assay. Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h. BTE showed cytotoxic effects against all carcinoma cell lines, however HT-29 and MCF-7 cells were more sensitive than A549. BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations. We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation. BTE induced also DNA strand breaks and oxidative damage to DNA in carcinoma cells HT-29 and MCF-7.

No MeSH data available.


Related in: MedlinePlus