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p38 MAP Kinase Inhibition Reduces Propionibacterium acnes-Induced Inflammation in Vitro.

Li WH, Zhang L, Lyte P, Rodriguez K, Cavender D, Southall MD - Dermatol Ther (Heidelb) (2015)

Bottom Line: Furthermore, SCIO-469, a selective inhibitor of p38α, showed similar effects in cultured keratinocytes.Topical treatment of SCIO-469 inhibited the P. acnes-induced phospho-p38 and cytokine secretion in human epidermal equivalents.Johnson & Johnson.

View Article: PubMed Central - PubMed

Affiliation: Department of Skin Biology and Pharmacology, The Johnson & Johnson Skin Research Center, Johnson & Johnson Consumer and Personal Products Worldwide, Division of Johnson and Johnson Consumer Companies, Inc., 199 Grandview Road, Skillman, NJ, 08558, USA, whli@its.jnj.com.

ABSTRACT

Introduction: Propionibacterium acnes, a ubiquitous skin bacterium, stimulates keratinocytes to produce a number of proinflammatory cytokines and may contribute to inflammatory acne. The aim of the study was to investigate whether P. acnes-induced proinflammatory cytokine release is mediated by P. acnes-induced activation of p38 mitogen-activated protein kinase (p38 MAPK or p38) in human keratinocytes.

Methods: Immunohistochemistry was used to evaluate p38 phosphorylation in human skin samples with or without acne. Primary human keratinocytes and epidermal skin equivalents were exposed to viable P. acnes. Phosphorylation of MAPKs without or with p38 inhibitors was examined by Western blot and cytokine secretion was detected by Enzyme-Linked Immunosorbent Assay (ELISA).

Results: Increased levels of phospho-p38 were observed in human acne lesions, predominantly in follicular and perifollicular keratinocytes. Exposure of cultured human keratinocytes to viable P. acnes resulted in phosphorylation of multiple members of the MAPK family, including rapid and transient activation of p38 and extracellular signal-related kinase (ERK1/2) and relatively slow but sustained activation of c-Jun N-terminal kinases (JNK1/2). Viable P. acnes induced the secretion of interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), and IL-8 from human keratinocytes. The phosphorylation of p38 (phospho-p38) and the secretion of cytokines induced by P. acnes in cultured keratinocytes were inhibited by SB203580, a p38α/β inhibitor. Furthermore, SCIO-469, a selective inhibitor of p38α, showed similar effects in cultured keratinocytes. Topical treatment of SCIO-469 inhibited the P. acnes-induced phospho-p38 and cytokine secretion in human epidermal equivalents.

Conclusion: The data demonstrate that P. acnes induces p38-dependent inflammatory responses in keratinocytes, and suggest that p38 may play an important role in the pathogenesis of inflammatory acne.

Funding: Johnson & Johnson.

No MeSH data available.


Related in: MedlinePlus

p38 is activated in human acne lesions. a Healthy human skin shows diffuse cytoplasmic staining in both epidermis and dermis. b High magnification insert of a illustrates weak nuclear staining in some hair follicle cells of a healthy skin. c Strong nuclear staining of phospho-p38 is found in follicular and perifollicular epidermis and dermis of a representative human inflammatory acne lesion. d High magnification insert of c demonstrates a solid dot pattern of nuclear staining phospho-p38. Bar 100 µm
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Fig1: p38 is activated in human acne lesions. a Healthy human skin shows diffuse cytoplasmic staining in both epidermis and dermis. b High magnification insert of a illustrates weak nuclear staining in some hair follicle cells of a healthy skin. c Strong nuclear staining of phospho-p38 is found in follicular and perifollicular epidermis and dermis of a representative human inflammatory acne lesion. d High magnification insert of c demonstrates a solid dot pattern of nuclear staining phospho-p38. Bar 100 µm

Mentions: Phospho-p38 expression was detected in all five acne tissue samples with a nuclear localization. A representative image of healthy skin shows mainly diffuse cytoplasmic staining of phospho-p38 in both epidermal and dermal cells (Fig. 1a), and weak nuclear staining in some hair follicle cells (Fig. 1b). In contrast, markedly increased level of phospho-p38 was observed in the inflammatory acne lesion (Fig. 1c). The phospho-p38 was mostly confined to the nuclei of follicular and perifollicular epidermal keratinocytes (Fig. 1d).Fig. 1


p38 MAP Kinase Inhibition Reduces Propionibacterium acnes-Induced Inflammation in Vitro.

Li WH, Zhang L, Lyte P, Rodriguez K, Cavender D, Southall MD - Dermatol Ther (Heidelb) (2015)

p38 is activated in human acne lesions. a Healthy human skin shows diffuse cytoplasmic staining in both epidermis and dermis. b High magnification insert of a illustrates weak nuclear staining in some hair follicle cells of a healthy skin. c Strong nuclear staining of phospho-p38 is found in follicular and perifollicular epidermis and dermis of a representative human inflammatory acne lesion. d High magnification insert of c demonstrates a solid dot pattern of nuclear staining phospho-p38. Bar 100 µm
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4374066&req=5

Fig1: p38 is activated in human acne lesions. a Healthy human skin shows diffuse cytoplasmic staining in both epidermis and dermis. b High magnification insert of a illustrates weak nuclear staining in some hair follicle cells of a healthy skin. c Strong nuclear staining of phospho-p38 is found in follicular and perifollicular epidermis and dermis of a representative human inflammatory acne lesion. d High magnification insert of c demonstrates a solid dot pattern of nuclear staining phospho-p38. Bar 100 µm
Mentions: Phospho-p38 expression was detected in all five acne tissue samples with a nuclear localization. A representative image of healthy skin shows mainly diffuse cytoplasmic staining of phospho-p38 in both epidermal and dermal cells (Fig. 1a), and weak nuclear staining in some hair follicle cells (Fig. 1b). In contrast, markedly increased level of phospho-p38 was observed in the inflammatory acne lesion (Fig. 1c). The phospho-p38 was mostly confined to the nuclei of follicular and perifollicular epidermal keratinocytes (Fig. 1d).Fig. 1

Bottom Line: Furthermore, SCIO-469, a selective inhibitor of p38α, showed similar effects in cultured keratinocytes.Topical treatment of SCIO-469 inhibited the P. acnes-induced phospho-p38 and cytokine secretion in human epidermal equivalents.Johnson & Johnson.

View Article: PubMed Central - PubMed

Affiliation: Department of Skin Biology and Pharmacology, The Johnson & Johnson Skin Research Center, Johnson & Johnson Consumer and Personal Products Worldwide, Division of Johnson and Johnson Consumer Companies, Inc., 199 Grandview Road, Skillman, NJ, 08558, USA, whli@its.jnj.com.

ABSTRACT

Introduction: Propionibacterium acnes, a ubiquitous skin bacterium, stimulates keratinocytes to produce a number of proinflammatory cytokines and may contribute to inflammatory acne. The aim of the study was to investigate whether P. acnes-induced proinflammatory cytokine release is mediated by P. acnes-induced activation of p38 mitogen-activated protein kinase (p38 MAPK or p38) in human keratinocytes.

Methods: Immunohistochemistry was used to evaluate p38 phosphorylation in human skin samples with or without acne. Primary human keratinocytes and epidermal skin equivalents were exposed to viable P. acnes. Phosphorylation of MAPKs without or with p38 inhibitors was examined by Western blot and cytokine secretion was detected by Enzyme-Linked Immunosorbent Assay (ELISA).

Results: Increased levels of phospho-p38 were observed in human acne lesions, predominantly in follicular and perifollicular keratinocytes. Exposure of cultured human keratinocytes to viable P. acnes resulted in phosphorylation of multiple members of the MAPK family, including rapid and transient activation of p38 and extracellular signal-related kinase (ERK1/2) and relatively slow but sustained activation of c-Jun N-terminal kinases (JNK1/2). Viable P. acnes induced the secretion of interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), and IL-8 from human keratinocytes. The phosphorylation of p38 (phospho-p38) and the secretion of cytokines induced by P. acnes in cultured keratinocytes were inhibited by SB203580, a p38α/β inhibitor. Furthermore, SCIO-469, a selective inhibitor of p38α, showed similar effects in cultured keratinocytes. Topical treatment of SCIO-469 inhibited the P. acnes-induced phospho-p38 and cytokine secretion in human epidermal equivalents.

Conclusion: The data demonstrate that P. acnes induces p38-dependent inflammatory responses in keratinocytes, and suggest that p38 may play an important role in the pathogenesis of inflammatory acne.

Funding: Johnson & Johnson.

No MeSH data available.


Related in: MedlinePlus