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Transcriptional analysis of micro-dissected articular cartilage in post-traumatic murine osteoarthritis.

Gardiner MD, Vincent TL, Driscoll C, Burleigh A, Bou-Gharios G, Saklatvala J, Nagase H, Chanalaris A - Osteoarthr. Cartil. (2014)

Bottom Line: Regulated genes decrease over time.Common genes include Jagged 1 (Jag1), Tetraspanin 2 (Tspan2), neuroblastoma, suppression of tumourigenicity 1 (Nbl1) and N-myc downstream regulated gene 2 (Ndrg2).The concomitant genes and pathways we identify may warrant further investigation as biomarkers or modulators of OA.

View Article: PubMed Central - PubMed

Affiliation: Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Old Road Campus, Roosevelt Drive, Headington, Oxford, OX3 7FY, UK. Electronic address: matthew.gardiner@kennedy.ox.ac.uk.

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Differentially expressed genes in the operated medial tibial cartilage at 2 (A) and 4 (B) weeks. The base 2 logarithm of the fold change for each gene that was confidently detected in our array was plotted against the base 2 logarithm of the P value that was adjusted for multiplicity. The names of the genes with a fold change greater than 2.8 or less than 0.35 are presented at 2 weeks, where the dotted vertical line represents the boundaries for those fold changes. At 4 weeks the significantly regulated genes with a fold change greater than 1.5 and less than 0.5 are represented.
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fig2: Differentially expressed genes in the operated medial tibial cartilage at 2 (A) and 4 (B) weeks. The base 2 logarithm of the fold change for each gene that was confidently detected in our array was plotted against the base 2 logarithm of the P value that was adjusted for multiplicity. The names of the genes with a fold change greater than 2.8 or less than 0.35 are presented at 2 weeks, where the dotted vertical line represents the boundaries for those fold changes. At 4 weeks the significantly regulated genes with a fold change greater than 1.5 and less than 0.5 are represented.

Mentions: At 2 weeks, 1224 genes (1425 probes) were differentially expressed [Fig. 2(A); Supplementary Table S3]. Keratocan (Kera) was the most significantly up-regulated gene, others included Mmp3, Mmp2 along with a further 14 matrix metalloproteinases (MMPs). Fibromodulin (Fmod) was one of a number of small leucine-rich repeat proteins (SLRPs) up-regulated. Myh4 was the most down-regulated gene, along with other contractile elements and many genes from the serpin family [Fig. 2(A)].


Transcriptional analysis of micro-dissected articular cartilage in post-traumatic murine osteoarthritis.

Gardiner MD, Vincent TL, Driscoll C, Burleigh A, Bou-Gharios G, Saklatvala J, Nagase H, Chanalaris A - Osteoarthr. Cartil. (2014)

Differentially expressed genes in the operated medial tibial cartilage at 2 (A) and 4 (B) weeks. The base 2 logarithm of the fold change for each gene that was confidently detected in our array was plotted against the base 2 logarithm of the P value that was adjusted for multiplicity. The names of the genes with a fold change greater than 2.8 or less than 0.35 are presented at 2 weeks, where the dotted vertical line represents the boundaries for those fold changes. At 4 weeks the significantly regulated genes with a fold change greater than 1.5 and less than 0.5 are represented.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4373757&req=5

fig2: Differentially expressed genes in the operated medial tibial cartilage at 2 (A) and 4 (B) weeks. The base 2 logarithm of the fold change for each gene that was confidently detected in our array was plotted against the base 2 logarithm of the P value that was adjusted for multiplicity. The names of the genes with a fold change greater than 2.8 or less than 0.35 are presented at 2 weeks, where the dotted vertical line represents the boundaries for those fold changes. At 4 weeks the significantly regulated genes with a fold change greater than 1.5 and less than 0.5 are represented.
Mentions: At 2 weeks, 1224 genes (1425 probes) were differentially expressed [Fig. 2(A); Supplementary Table S3]. Keratocan (Kera) was the most significantly up-regulated gene, others included Mmp3, Mmp2 along with a further 14 matrix metalloproteinases (MMPs). Fibromodulin (Fmod) was one of a number of small leucine-rich repeat proteins (SLRPs) up-regulated. Myh4 was the most down-regulated gene, along with other contractile elements and many genes from the serpin family [Fig. 2(A)].

Bottom Line: Regulated genes decrease over time.Common genes include Jagged 1 (Jag1), Tetraspanin 2 (Tspan2), neuroblastoma, suppression of tumourigenicity 1 (Nbl1) and N-myc downstream regulated gene 2 (Ndrg2).The concomitant genes and pathways we identify may warrant further investigation as biomarkers or modulators of OA.

View Article: PubMed Central - PubMed

Affiliation: Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Old Road Campus, Roosevelt Drive, Headington, Oxford, OX3 7FY, UK. Electronic address: matthew.gardiner@kennedy.ox.ac.uk.

Show MeSH
Related in: MedlinePlus