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"On-off" thermoresponsive coating agent containing salicylic acid applied to maize seeds for chilling tolerance.

Guan Y, Li Z, He F, Huang Y, Song W, Hu J - PLoS ONE (2015)

Bottom Line: The results showed that the hydrogel obtained a phase transition temperature near 12°C with a NIPAM to MBA weight ratio of 1: 0.1988 (w/w).In addition, when seedlings of both maize varieties suffered a short chilling stress (5°C), higher concentrations of SA-loaded hydrogel resulted in increased germination energy, germination percentage, germination index, root length, shoot height, dry weight of roots and shoots and protective enzyme activities and a decreased malondialdehyde content in coated maize seeds compared to single SA treatments.The majority of these physiological and biochemical parameters achieved significant levels compared with the control.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China.

ABSTRACT
Chilling stress is an important constraint for maize seed establishment in the field. In this study, a type of "on-off" thermoresponsive coating agent containing poly (N-isopropylacrylamide-co-butylmethacrylate) (Abbr. P(NIPAm-co-BMA)) hydrogel was developed to improve the chilling tolerance of coated maize seed. The P(NIPAm-co-BMA) hydrogel was synthesized by free-radical polymerization of N-isopropylacrylamide (NIPAm) and butylmethacrylate (BMA). Salicylic acid (SA) was loaded in the hydrogel as the chilling resistance agent. SA-loaded P(NIPAm-co-BMA) was used for seed film-coating of two maize varieties, Huang C (HC, chilling-tolerant) and Mo17 (chilling-sensitive), to investigate the coated seed germination and seedling growth status under chilling stress. The results showed that the hydrogel obtained a phase transition temperature near 12°C with a NIPAM to MBA weight ratio of 1: 0.1988 (w/w). The temperature of 12°C was considered the "on-off" temperature for chilling-resistant agent release; the SA was released from the hydrogel more rapidly at external temperatures below 12°C than above 12°C. In addition, when seedlings of both maize varieties suffered a short chilling stress (5°C), higher concentrations of SA-loaded hydrogel resulted in increased germination energy, germination percentage, germination index, root length, shoot height, dry weight of roots and shoots and protective enzyme activities and a decreased malondialdehyde content in coated maize seeds compared to single SA treatments. The majority of these physiological and biochemical parameters achieved significant levels compared with the control. Therefore, SA-loaded P(NIPAm-co-BMA), a nontoxic thermoresponsive hydrogel, can be used as an effective material for chilling tolerance in film-coated maize seeds.

No MeSH data available.


Related in: MedlinePlus

The effect of pH on SA release from the P(NIPAm-co-BMA) hydrogel under circulative pulse temperatures.P(NIPAm-co-BMA) is poly (N-isopropyl acrylamide-co-butylmethacrylate) and SA is salicylic acid. The pulsatile SA releases from the hydrogel under phosphate buffer solutions with different pH values (pH = 5, 7, 9). The circulative pulse temperature changes between 9°C and 15°C every 15 min. Error bars represent ±S.E.
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pone.0120695.g006: The effect of pH on SA release from the P(NIPAm-co-BMA) hydrogel under circulative pulse temperatures.P(NIPAm-co-BMA) is poly (N-isopropyl acrylamide-co-butylmethacrylate) and SA is salicylic acid. The pulsatile SA releases from the hydrogel under phosphate buffer solutions with different pH values (pH = 5, 7, 9). The circulative pulse temperature changes between 9°C and 15°C every 15 min. Error bars represent ±S.E.

Mentions: In addition, the SA release ratio patterns under circulative pulse temperatures in three PBS buffers with different pH values were similar (Fig. 6). Almost 7.0% of the SA was released from the P(NIPAm-co-BMA) hydrogel every 15 min at 9°C; however, only 3.0% of the SA was released at 15°C.


"On-off" thermoresponsive coating agent containing salicylic acid applied to maize seeds for chilling tolerance.

Guan Y, Li Z, He F, Huang Y, Song W, Hu J - PLoS ONE (2015)

The effect of pH on SA release from the P(NIPAm-co-BMA) hydrogel under circulative pulse temperatures.P(NIPAm-co-BMA) is poly (N-isopropyl acrylamide-co-butylmethacrylate) and SA is salicylic acid. The pulsatile SA releases from the hydrogel under phosphate buffer solutions with different pH values (pH = 5, 7, 9). The circulative pulse temperature changes between 9°C and 15°C every 15 min. Error bars represent ±S.E.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4373755&req=5

pone.0120695.g006: The effect of pH on SA release from the P(NIPAm-co-BMA) hydrogel under circulative pulse temperatures.P(NIPAm-co-BMA) is poly (N-isopropyl acrylamide-co-butylmethacrylate) and SA is salicylic acid. The pulsatile SA releases from the hydrogel under phosphate buffer solutions with different pH values (pH = 5, 7, 9). The circulative pulse temperature changes between 9°C and 15°C every 15 min. Error bars represent ±S.E.
Mentions: In addition, the SA release ratio patterns under circulative pulse temperatures in three PBS buffers with different pH values were similar (Fig. 6). Almost 7.0% of the SA was released from the P(NIPAm-co-BMA) hydrogel every 15 min at 9°C; however, only 3.0% of the SA was released at 15°C.

Bottom Line: The results showed that the hydrogel obtained a phase transition temperature near 12°C with a NIPAM to MBA weight ratio of 1: 0.1988 (w/w).In addition, when seedlings of both maize varieties suffered a short chilling stress (5°C), higher concentrations of SA-loaded hydrogel resulted in increased germination energy, germination percentage, germination index, root length, shoot height, dry weight of roots and shoots and protective enzyme activities and a decreased malondialdehyde content in coated maize seeds compared to single SA treatments.The majority of these physiological and biochemical parameters achieved significant levels compared with the control.

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China.

ABSTRACT
Chilling stress is an important constraint for maize seed establishment in the field. In this study, a type of "on-off" thermoresponsive coating agent containing poly (N-isopropylacrylamide-co-butylmethacrylate) (Abbr. P(NIPAm-co-BMA)) hydrogel was developed to improve the chilling tolerance of coated maize seed. The P(NIPAm-co-BMA) hydrogel was synthesized by free-radical polymerization of N-isopropylacrylamide (NIPAm) and butylmethacrylate (BMA). Salicylic acid (SA) was loaded in the hydrogel as the chilling resistance agent. SA-loaded P(NIPAm-co-BMA) was used for seed film-coating of two maize varieties, Huang C (HC, chilling-tolerant) and Mo17 (chilling-sensitive), to investigate the coated seed germination and seedling growth status under chilling stress. The results showed that the hydrogel obtained a phase transition temperature near 12°C with a NIPAM to MBA weight ratio of 1: 0.1988 (w/w). The temperature of 12°C was considered the "on-off" temperature for chilling-resistant agent release; the SA was released from the hydrogel more rapidly at external temperatures below 12°C than above 12°C. In addition, when seedlings of both maize varieties suffered a short chilling stress (5°C), higher concentrations of SA-loaded hydrogel resulted in increased germination energy, germination percentage, germination index, root length, shoot height, dry weight of roots and shoots and protective enzyme activities and a decreased malondialdehyde content in coated maize seeds compared to single SA treatments. The majority of these physiological and biochemical parameters achieved significant levels compared with the control. Therefore, SA-loaded P(NIPAm-co-BMA), a nontoxic thermoresponsive hydrogel, can be used as an effective material for chilling tolerance in film-coated maize seeds.

No MeSH data available.


Related in: MedlinePlus