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Understanding Thiel embalming in pig kidneys to develop a new circulation model.

Willaert W, De Vos M, Van Hoof T, Delrue L, Pattyn P, D'Herde K - PLoS ONE (2015)

Bottom Line: The kidneys in both groups were then immersed in concentrated salt solutions to reduce their weight and volume.There were no increases in weight (P = 0.26) and volume (P = 0.79); and pressure further decreased (P = 0.032) after more than 60 minutes of reperfusion with paraffinum perliquidum, whereas there were increases in weight (P = 0.005), volume (P = 0.032) and pressure (P < 0.0001) after reperfusion with diluted polyethylene glycol.Prolonged vascular reperfusion with paraffinum perliquidum can be established in this model without increases in weight, volume and pressure.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, Ghent University Hospital, Ghent, Belgium.

ABSTRACT
The quality of tissue preservation in Thiel embalmed bodies varies. Research on the administered embalming volume and its vascular distribution may elucidate one of the mechanisms of tissue preservation and allow for new applications of Thiel embalming. Vascular embalming with (group 1, n = 15) or without (group 2, n = 20) contrast agent was initiated in pig kidneys. The distribution of Thiel embalming solution in group 1 was visualized using computed tomography. The kidneys in both groups were then immersed in concentrated salt solutions to reduce their weight and volume. Afterwards, to mimic a lifelike circulation in the vessels, group 2 underwent pump-driven reperfusion for 120 minutes with either paraffinum perliquidum or diluted polyethylene glycol. The circulation was imaged with computed tomography. All of the kidneys were adequately preserved. The embalming solution spread diffusely in the kidney, but fluid accumulation was present. Subsequent immersion in concentrated salt solutions reduced weight (P < 0.01) and volume (P < 0.01). Reperfusion for 120 minutes was established in group 2. Paraffinum perliquidum filled both major vessels and renal tissue, whereas diluted polyethylene glycol spread widely in the kidney. There were no increases in weight (P = 0.26) and volume (P = 0.79); and pressure further decreased (P = 0.032) after more than 60 minutes of reperfusion with paraffinum perliquidum, whereas there were increases in weight (P = 0.005), volume (P = 0.032) and pressure (P < 0.0001) after reperfusion with diluted polyethylene glycol. Arterial embalming of kidneys results in successful preservation due to complete parenchymatous spreading. More research is needed to determine whether other factors affect embalming quality. Dehydration is an effective method to regain the organs' initial status. Prolonged vascular reperfusion with paraffinum perliquidum can be established in this model without increases in weight, volume and pressure.

No MeSH data available.


Related in: MedlinePlus

Pressure-controlled embalming of pig kidneys.Thiel embalming fluid is pumped in the renal artery, and a mixture of blood and/or embalming fluid eventually leaves the kidney through the renal vein.
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pone.0120114.g001: Pressure-controlled embalming of pig kidneys.Thiel embalming fluid is pumped in the renal artery, and a mixture of blood and/or embalming fluid eventually leaves the kidney through the renal vein.

Mentions: After initiating the embalming procedure, the renal artery was punctured with a BD Insyte-W 22-gauge catheter (BD Vialon, Madrid, Spain) and connected to an ultraminiature fibre optic pressure transducer (Samba 201 CAP, Harvard Apparatus, Cedex, France). The maximum intra-arterial pressure allowed was 65 mmHg, in agreement with the in vivo arterial blood pressure of pigs (Fig. 1). The type of venous drainage (transparent, serosanguinous or sanguinous) was assessed at the end of the embalming procedure. Subsequently, the weight, volume and swelling of the kidneys were noted. The vascular spreading of the embalming product was imaged by computed tomography (CT; Somatom Definition Flash, Siemens Healthcare Sector, Forchheim, Germany).


Understanding Thiel embalming in pig kidneys to develop a new circulation model.

Willaert W, De Vos M, Van Hoof T, Delrue L, Pattyn P, D'Herde K - PLoS ONE (2015)

Pressure-controlled embalming of pig kidneys.Thiel embalming fluid is pumped in the renal artery, and a mixture of blood and/or embalming fluid eventually leaves the kidney through the renal vein.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4373718&req=5

pone.0120114.g001: Pressure-controlled embalming of pig kidneys.Thiel embalming fluid is pumped in the renal artery, and a mixture of blood and/or embalming fluid eventually leaves the kidney through the renal vein.
Mentions: After initiating the embalming procedure, the renal artery was punctured with a BD Insyte-W 22-gauge catheter (BD Vialon, Madrid, Spain) and connected to an ultraminiature fibre optic pressure transducer (Samba 201 CAP, Harvard Apparatus, Cedex, France). The maximum intra-arterial pressure allowed was 65 mmHg, in agreement with the in vivo arterial blood pressure of pigs (Fig. 1). The type of venous drainage (transparent, serosanguinous or sanguinous) was assessed at the end of the embalming procedure. Subsequently, the weight, volume and swelling of the kidneys were noted. The vascular spreading of the embalming product was imaged by computed tomography (CT; Somatom Definition Flash, Siemens Healthcare Sector, Forchheim, Germany).

Bottom Line: The kidneys in both groups were then immersed in concentrated salt solutions to reduce their weight and volume.There were no increases in weight (P = 0.26) and volume (P = 0.79); and pressure further decreased (P = 0.032) after more than 60 minutes of reperfusion with paraffinum perliquidum, whereas there were increases in weight (P = 0.005), volume (P = 0.032) and pressure (P < 0.0001) after reperfusion with diluted polyethylene glycol.Prolonged vascular reperfusion with paraffinum perliquidum can be established in this model without increases in weight, volume and pressure.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, Ghent University Hospital, Ghent, Belgium.

ABSTRACT
The quality of tissue preservation in Thiel embalmed bodies varies. Research on the administered embalming volume and its vascular distribution may elucidate one of the mechanisms of tissue preservation and allow for new applications of Thiel embalming. Vascular embalming with (group 1, n = 15) or without (group 2, n = 20) contrast agent was initiated in pig kidneys. The distribution of Thiel embalming solution in group 1 was visualized using computed tomography. The kidneys in both groups were then immersed in concentrated salt solutions to reduce their weight and volume. Afterwards, to mimic a lifelike circulation in the vessels, group 2 underwent pump-driven reperfusion for 120 minutes with either paraffinum perliquidum or diluted polyethylene glycol. The circulation was imaged with computed tomography. All of the kidneys were adequately preserved. The embalming solution spread diffusely in the kidney, but fluid accumulation was present. Subsequent immersion in concentrated salt solutions reduced weight (P < 0.01) and volume (P < 0.01). Reperfusion for 120 minutes was established in group 2. Paraffinum perliquidum filled both major vessels and renal tissue, whereas diluted polyethylene glycol spread widely in the kidney. There were no increases in weight (P = 0.26) and volume (P = 0.79); and pressure further decreased (P = 0.032) after more than 60 minutes of reperfusion with paraffinum perliquidum, whereas there were increases in weight (P = 0.005), volume (P = 0.032) and pressure (P < 0.0001) after reperfusion with diluted polyethylene glycol. Arterial embalming of kidneys results in successful preservation due to complete parenchymatous spreading. More research is needed to determine whether other factors affect embalming quality. Dehydration is an effective method to regain the organs' initial status. Prolonged vascular reperfusion with paraffinum perliquidum can be established in this model without increases in weight, volume and pressure.

No MeSH data available.


Related in: MedlinePlus