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In vivo and in vitro assessment of brain bioenergetics in aging rats.

Vančová O, Bačiak L, Kašparová S, Kucharská J, Palacios HH, Horecký J, Aliev G - J. Cell. Mol. Med. (2010)

Bottom Line: In vivo brain bioenergetics were measured using ³¹P nuclear magnetic resonance (NMR) spectroscopy and in vitro by polarographic analysis of mitochondrial oxidative phosphorylation.When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo.We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.

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Percentual changes of in vitro measured OXPHOS parameters in brain mitochondria from young (3 months old), adult (17 months old) and aged (36 months old) male Wistar rats. (A) Complex I–sodium glutamate / malic acid NAD substrate, (B) Complex II–succinate FAD substrate (respectively, A and B). ***P < 0.001, **P < 0.01, *P < 0.05 versus 3 months old rats. ++P < 0.01, +P < 0.05 versus 17 months old rats.
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fig02: Percentual changes of in vitro measured OXPHOS parameters in brain mitochondria from young (3 months old), adult (17 months old) and aged (36 months old) male Wistar rats. (A) Complex I–sodium glutamate / malic acid NAD substrate, (B) Complex II–succinate FAD substrate (respectively, A and B). ***P < 0.001, **P < 0.01, *P < 0.05 versus 3 months old rats. ++P < 0.01, +P < 0.05 versus 17 months old rats.

Mentions: Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.


In vivo and in vitro assessment of brain bioenergetics in aging rats.

Vančová O, Bačiak L, Kašparová S, Kucharská J, Palacios HH, Horecký J, Aliev G - J. Cell. Mol. Med. (2010)

Percentual changes of in vitro measured OXPHOS parameters in brain mitochondria from young (3 months old), adult (17 months old) and aged (36 months old) male Wistar rats. (A) Complex I–sodium glutamate / malic acid NAD substrate, (B) Complex II–succinate FAD substrate (respectively, A and B). ***P < 0.001, **P < 0.01, *P < 0.05 versus 3 months old rats. ++P < 0.01, +P < 0.05 versus 17 months old rats.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373491&req=5

fig02: Percentual changes of in vitro measured OXPHOS parameters in brain mitochondria from young (3 months old), adult (17 months old) and aged (36 months old) male Wistar rats. (A) Complex I–sodium glutamate / malic acid NAD substrate, (B) Complex II–succinate FAD substrate (respectively, A and B). ***P < 0.001, **P < 0.01, *P < 0.05 versus 3 months old rats. ++P < 0.01, +P < 0.05 versus 17 months old rats.
Mentions: Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.

Bottom Line: In vivo brain bioenergetics were measured using ³¹P nuclear magnetic resonance (NMR) spectroscopy and in vitro by polarographic analysis of mitochondrial oxidative phosphorylation.When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo.We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.

Show MeSH
Related in: MedlinePlus