In vivo and in vitro assessment of brain bioenergetics in aging rats.
Bottom Line: In vivo brain bioenergetics were measured using ³¹P nuclear magnetic resonance (NMR) spectroscopy and in vitro by polarographic analysis of mitochondrial oxidative phosphorylation.When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo.We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.
Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.Show MeSH
Related in: MedlinePlus
Mentions: Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.
Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.