In vivo and in vitro assessment of brain bioenergetics in aging rats.
Bottom Line: When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo.The levels of enzymatic antioxidants catalase, GPx and GST significantly decreased in the brain tissue as well as in the peripheral blood of aged rats.We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.
Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.Show MeSH
Related in: MedlinePlus
Mentions: Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.
Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.