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In vivo and in vitro assessment of brain bioenergetics in aging rats.

Vančová O, Bačiak L, Kašparová S, Kucharská J, Palacios HH, Horecký J, Aliev G - J. Cell. Mol. Med. (2010)

Bottom Line: In vivo brain bioenergetics were measured using ³¹P nuclear magnetic resonance (NMR) spectroscopy and in vitro by polarographic analysis of mitochondrial oxidative phosphorylation.When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo.We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.

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Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.
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fig01: Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.

Mentions: Relative concentrations of phosphate metabolism were determined from integrals of their signal in 31P MR spectra using program MESTREC-C 1.5.1 (Table 1 and Fig. 1). Time-dependent 31P MRS saturation transfer was applied to determine the pseudo-first-order rate constant of forward CK reaction (kfor). While the analysis of steady-state 31P MR spectra revealed significant increase in the ratio of PCr/ATP (P < 0.001), PCr/Pi (P < 0.01) and PME/ATP (P < 0.05), we found significant (P < 0.001) decrease of forward kfor in aged rats as compared to the young group.


In vivo and in vitro assessment of brain bioenergetics in aging rats.

Vančová O, Bačiak L, Kašparová S, Kucharská J, Palacios HH, Horecký J, Aliev G - J. Cell. Mol. Med. (2010)

Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373491&req=5

fig01: Changes of brain bioenergetics measured in vivo by 31P MRS in aged (36 months old) compared to young (3 months old) male Wistar rats. MRS, magnetic resonance spectroscopy; PCr/ATP, phosphocreatine to adenosine three phosphate ratio; PCr/Pi, PCr to inorganic phosphate ratio; PME/ATP, phosphate monoester to ATP ratio; kfor, forward rate constant of CK and pHi, intracellular pH. ***P < 0.001, **P < 0.01, *P < 0.05.
Mentions: Relative concentrations of phosphate metabolism were determined from integrals of their signal in 31P MR spectra using program MESTREC-C 1.5.1 (Table 1 and Fig. 1). Time-dependent 31P MRS saturation transfer was applied to determine the pseudo-first-order rate constant of forward CK reaction (kfor). While the analysis of steady-state 31P MR spectra revealed significant increase in the ratio of PCr/ATP (P < 0.001), PCr/Pi (P < 0.01) and PME/ATP (P < 0.05), we found significant (P < 0.001) decrease of forward kfor in aged rats as compared to the young group.

Bottom Line: In vivo brain bioenergetics were measured using ³¹P nuclear magnetic resonance (NMR) spectroscopy and in vitro by polarographic analysis of mitochondrial oxidative phosphorylation.When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo.We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.

Show MeSH
Related in: MedlinePlus