In vivo and in vitro assessment of brain bioenergetics in aging rats.
Bottom Line: When compared to the young controls, a significant decrease of age-dependent mitochondrial respiration and adenosine-3-phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo.The levels of enzymatic antioxidants catalase, GPx and GST significantly decreased in the brain tissue as well as in the peripheral blood of aged rats.We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age-related disorders of brain energy metabolism.
Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.Show MeSH
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Mentions: Relative concentrations of phosphate metabolism were determined from integrals of their signal in 31P MR spectra using program MESTREC-C 1.5.1 (Table 1 and Fig. 1). Time-dependent 31P MRS saturation transfer was applied to determine the pseudo-first-order rate constant of forward CK reaction (kfor). While the analysis of steady-state 31P MR spectra revealed significant increase in the ratio of PCr/ATP (P < 0.001), PCr/Pi (P < 0.01) and PME/ATP (P < 0.05), we found significant (P < 0.001) decrease of forward kfor in aged rats as compared to the young group.
Affiliation: Pharmacobiochemical Laboratory, Comenius University School of Medicine, Bratislava, Slovakia.