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Cardiac telocytes: serial dynamic images in cell culture.

Suciu L, Nicolescu MI, Popescu LM - J. Cell. Mol. Med. (2010)

Bottom Line: We used a protocol that favoured interstitial cell selection from adult mouse myocardium.Videomicroscopy showed dynamic interactions of neighbour TC during the network formation.During their movement, TC leave behind distal segments (podomeres) of their Tp as guiding marks for the neighbouring cells to follow during network rearrangement.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, 'Carol Davila' University of Medicine and Pharmacy, Bucharest, Romania.

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Related in: MedlinePlus

Serial snapshots selected from a time-lapse videomicroscopy recording of adult mouse myocardial interstitial cell culture. The chronological order of sequences is indicated by white arrowheads at the edge of each frame. TC were designated a, b, c and d in order of appearance in the microscopic field. They start structuring a network. The a TC (yellow) is the most active cell, scouting the area and planting marks (yellow arrowheads) out of its own regressing Tp (yellow arrows), for the other cells to follow. TC b2* and c are using those retracting Tp as guiding wires to grow their own cell projections (black and blue arrows). Following marks left by TC a (enclosed areas and yellow arrowheads), TC c leaves an entire Tp (black arrowhead) as a guiding mark for b2* and upcoming d cell. Note: a and b cells undergo cell division; daughter cells were assigned numeric indices.
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fig01: Serial snapshots selected from a time-lapse videomicroscopy recording of adult mouse myocardial interstitial cell culture. The chronological order of sequences is indicated by white arrowheads at the edge of each frame. TC were designated a, b, c and d in order of appearance in the microscopic field. They start structuring a network. The a TC (yellow) is the most active cell, scouting the area and planting marks (yellow arrowheads) out of its own regressing Tp (yellow arrows), for the other cells to follow. TC b2* and c are using those retracting Tp as guiding wires to grow their own cell projections (black and blue arrows). Following marks left by TC a (enclosed areas and yellow arrowheads), TC c leaves an entire Tp (black arrowhead) as a guiding mark for b2* and upcoming d cell. Note: a and b cells undergo cell division; daughter cells were assigned numeric indices.

Mentions: Figure 1 shows that some cells in the 48-hr-old culture, which correspond to the TC morphological profile, extend very long and thin Tp that reach out and temporarily interconnect with similar cells in a primary network-like layout. Cell culture confirms repetitive Tp structure shown in electron microscopy (EM) studies [1], of alternating thin segments (podomeres) and dilations (podoms). The mobility, as well as the plasticity of Tp, is based on this moniliform ‘design’.


Cardiac telocytes: serial dynamic images in cell culture.

Suciu L, Nicolescu MI, Popescu LM - J. Cell. Mol. Med. (2010)

Serial snapshots selected from a time-lapse videomicroscopy recording of adult mouse myocardial interstitial cell culture. The chronological order of sequences is indicated by white arrowheads at the edge of each frame. TC were designated a, b, c and d in order of appearance in the microscopic field. They start structuring a network. The a TC (yellow) is the most active cell, scouting the area and planting marks (yellow arrowheads) out of its own regressing Tp (yellow arrows), for the other cells to follow. TC b2* and c are using those retracting Tp as guiding wires to grow their own cell projections (black and blue arrows). Following marks left by TC a (enclosed areas and yellow arrowheads), TC c leaves an entire Tp (black arrowhead) as a guiding mark for b2* and upcoming d cell. Note: a and b cells undergo cell division; daughter cells were assigned numeric indices.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373489&req=5

fig01: Serial snapshots selected from a time-lapse videomicroscopy recording of adult mouse myocardial interstitial cell culture. The chronological order of sequences is indicated by white arrowheads at the edge of each frame. TC were designated a, b, c and d in order of appearance in the microscopic field. They start structuring a network. The a TC (yellow) is the most active cell, scouting the area and planting marks (yellow arrowheads) out of its own regressing Tp (yellow arrows), for the other cells to follow. TC b2* and c are using those retracting Tp as guiding wires to grow their own cell projections (black and blue arrows). Following marks left by TC a (enclosed areas and yellow arrowheads), TC c leaves an entire Tp (black arrowhead) as a guiding mark for b2* and upcoming d cell. Note: a and b cells undergo cell division; daughter cells were assigned numeric indices.
Mentions: Figure 1 shows that some cells in the 48-hr-old culture, which correspond to the TC morphological profile, extend very long and thin Tp that reach out and temporarily interconnect with similar cells in a primary network-like layout. Cell culture confirms repetitive Tp structure shown in electron microscopy (EM) studies [1], of alternating thin segments (podomeres) and dilations (podoms). The mobility, as well as the plasticity of Tp, is based on this moniliform ‘design’.

Bottom Line: We used a protocol that favoured interstitial cell selection from adult mouse myocardium.Videomicroscopy showed dynamic interactions of neighbour TC during the network formation.During their movement, TC leave behind distal segments (podomeres) of their Tp as guiding marks for the neighbouring cells to follow during network rearrangement.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, 'Carol Davila' University of Medicine and Pharmacy, Bucharest, Romania.

Show MeSH
Related in: MedlinePlus