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Affinity pulldown of γ-secretase and associated proteins from human and rat brain.

Teranishi Y, Hur JY, Welander H, Frånberg J, Aoki M, Winblad B, Frykman S, Tjernberg LO - J. Cell. Mol. Med. (2010)

Bottom Line: After pulldown using streptavidin beads, bound proteins were eluted under reducing conditions and digested by trypsin.Interestingly, TMP21 and the PS associated protein syntaxin1 were associated to γ-secretase in rat brain.We suggest that the present method can be used for further studies on the composition of the γ-secretase complex.

View Article: PubMed Central - PubMed

Affiliation: The Karolinska Institutet (KI) Dainippon Sumitomo Pharma Alzheimer Center (KASPAC), KI-Alzheimer's Disease Research Center, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Novum, Huddinge, Sweden.

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Summary of identified proteins from rat brain. (A) A total of 91 proteins were uniquely identified from samples in the absence of L-685,458 after subtraction. The subtraction analysis was performed based on statistically significant peptides data. (B) Distribution of the relative number of hydrophobic (integral membrane proteins and membrane associated proteins) and soluble proteins. (C) Identified proteins were classified in three categories. Reported proteins were identified only in the absence of competing inhibitor. The number in parenthesis represents MASCOT score.
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fig05: Summary of identified proteins from rat brain. (A) A total of 91 proteins were uniquely identified from samples in the absence of L-685,458 after subtraction. The subtraction analysis was performed based on statistically significant peptides data. (B) Distribution of the relative number of hydrophobic (integral membrane proteins and membrane associated proteins) and soluble proteins. (C) Identified proteins were classified in three categories. Reported proteins were identified only in the absence of competing inhibitor. The number in parenthesis represents MASCOT score.

Mentions: In total, 91 proteins were uniquely identified in the sample without competing inhibitor. Most of these were integral membrane proteins (49%) or membrane associated proteins (29%) according to the annotation in UniProtKB/SwissProt database (Fig. 5). These proteins are presently evaluated with respect to association and their effect on Aβ40 and Aβ42 production.


Affinity pulldown of γ-secretase and associated proteins from human and rat brain.

Teranishi Y, Hur JY, Welander H, Frånberg J, Aoki M, Winblad B, Frykman S, Tjernberg LO - J. Cell. Mol. Med. (2010)

Summary of identified proteins from rat brain. (A) A total of 91 proteins were uniquely identified from samples in the absence of L-685,458 after subtraction. The subtraction analysis was performed based on statistically significant peptides data. (B) Distribution of the relative number of hydrophobic (integral membrane proteins and membrane associated proteins) and soluble proteins. (C) Identified proteins were classified in three categories. Reported proteins were identified only in the absence of competing inhibitor. The number in parenthesis represents MASCOT score.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373488&req=5

fig05: Summary of identified proteins from rat brain. (A) A total of 91 proteins were uniquely identified from samples in the absence of L-685,458 after subtraction. The subtraction analysis was performed based on statistically significant peptides data. (B) Distribution of the relative number of hydrophobic (integral membrane proteins and membrane associated proteins) and soluble proteins. (C) Identified proteins were classified in three categories. Reported proteins were identified only in the absence of competing inhibitor. The number in parenthesis represents MASCOT score.
Mentions: In total, 91 proteins were uniquely identified in the sample without competing inhibitor. Most of these were integral membrane proteins (49%) or membrane associated proteins (29%) according to the annotation in UniProtKB/SwissProt database (Fig. 5). These proteins are presently evaluated with respect to association and their effect on Aβ40 and Aβ42 production.

Bottom Line: After pulldown using streptavidin beads, bound proteins were eluted under reducing conditions and digested by trypsin.Interestingly, TMP21 and the PS associated protein syntaxin1 were associated to γ-secretase in rat brain.We suggest that the present method can be used for further studies on the composition of the γ-secretase complex.

View Article: PubMed Central - PubMed

Affiliation: The Karolinska Institutet (KI) Dainippon Sumitomo Pharma Alzheimer Center (KASPAC), KI-Alzheimer's Disease Research Center, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Novum, Huddinge, Sweden.

Show MeSH
Related in: MedlinePlus