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Telocytes accompanying cardiomyocyte in primary culture: two- and three-dimensional culture environment.

Zhou J, Zhang Y, Wen X, Cao J, Li D, Lin Q, Wang H, Liu Z, Duan C, Wu K, Wang C - J. Cell. Mol. Med. (2010)

Bottom Line: Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium.The results showed that typical telocytes are distributed among cardiomyocytes, connecting them by long telopodes.Also, a significant number of vimentin+ telocytes were present within engineered heart tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Republic of China.

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Methylene blue staining of cells in primary culture at day 10. (A) Telocytes (arrow) with long and extremely thin prolongations, telopodes. (B) Telocytes (arrows) involved in the consistent beating ‘syncytium’. Bar = 50 μm.
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fig05: Methylene blue staining of cells in primary culture at day 10. (A) Telocytes (arrow) with long and extremely thin prolongations, telopodes. (B) Telocytes (arrows) involved in the consistent beating ‘syncytium’. Bar = 50 μm.

Mentions: Methylene blue staining further confirmed the existence of telocytes in primary culture. We used supravital methylene blue staining to identify telocytes in vitro, as recommended in [12–14]. Methylene blue staining showed that telocytes cultured in vitro had long and extremely thin prolongations – telopodes (Fig. 5A). They connected neighbouring cardiac cells making a consistent beating ‘syncytium’ (Fig. 5B).


Telocytes accompanying cardiomyocyte in primary culture: two- and three-dimensional culture environment.

Zhou J, Zhang Y, Wen X, Cao J, Li D, Lin Q, Wang H, Liu Z, Duan C, Wu K, Wang C - J. Cell. Mol. Med. (2010)

Methylene blue staining of cells in primary culture at day 10. (A) Telocytes (arrow) with long and extremely thin prolongations, telopodes. (B) Telocytes (arrows) involved in the consistent beating ‘syncytium’. Bar = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373485&req=5

fig05: Methylene blue staining of cells in primary culture at day 10. (A) Telocytes (arrow) with long and extremely thin prolongations, telopodes. (B) Telocytes (arrows) involved in the consistent beating ‘syncytium’. Bar = 50 μm.
Mentions: Methylene blue staining further confirmed the existence of telocytes in primary culture. We used supravital methylene blue staining to identify telocytes in vitro, as recommended in [12–14]. Methylene blue staining showed that telocytes cultured in vitro had long and extremely thin prolongations – telopodes (Fig. 5A). They connected neighbouring cardiac cells making a consistent beating ‘syncytium’ (Fig. 5B).

Bottom Line: Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium.The results showed that typical telocytes are distributed among cardiomyocytes, connecting them by long telopodes.Also, a significant number of vimentin+ telocytes were present within engineered heart tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Republic of China.

Show MeSH