Telocytes accompanying cardiomyocyte in primary culture: two- and three-dimensional culture environment.
Bottom Line: Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium.The results showed that typical telocytes are distributed among cardiomyocytes, connecting them by long telopodes.Also, a significant number of vimentin+ telocytes were present within engineered heart tissue.
Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Republic of China.Show MeSH
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Mentions: In primary culture of cardiomyocytes isolated from myocardial tissues, the typical telocytes were detected. They have a fusiform cell body and two long prolongations (Fig. 1A). The morphology of telocytes in vitro is different from cardiac cells. After 7 days of culture, typical morphological features of telocytes appeared under light microscope: a small oval-shaped cell body with extremely long and thin moniliform prolongations (telopodes) extended from the cell body (Figs. 1 and 2). Telocytes often accompanied the cardiomyocytes and their telopodes ended up on cardiomyocytes. However, telocytes with their long prolongations interconnected cardiomyocytes and accelerated their synchronously beating (Figs. 3 and 4 and Movie S1).
Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Republic of China.