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Telocytes accompanying cardiomyocyte in primary culture: two- and three-dimensional culture environment.

Zhou J, Zhang Y, Wen X, Cao J, Li D, Lin Q, Wang H, Liu Z, Duan C, Wu K, Wang C - J. Cell. Mol. Med. (2010)

Bottom Line: Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium.The results showed that typical telocytes are distributed among cardiomyocytes, connecting them by long telopodes.Also, a significant number of vimentin+ telocytes were present within engineered heart tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Republic of China.

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Telocytes in primary culture at day 7. (A) Typical telocytes with fusiform cell body and two long prolongations (telopodes). (B) Telocytes with two or three prolongations were different from cardiac cells. Bar = 50 μm.
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fig01: Telocytes in primary culture at day 7. (A) Typical telocytes with fusiform cell body and two long prolongations (telopodes). (B) Telocytes with two or three prolongations were different from cardiac cells. Bar = 50 μm.

Mentions: In primary culture of cardiomyocytes isolated from myocardial tissues, the typical telocytes were detected. They have a fusiform cell body and two long prolongations (Fig. 1A). The morphology of telocytes in vitro is different from cardiac cells. After 7 days of culture, typical morphological features of telocytes appeared under light microscope: a small oval-shaped cell body with extremely long and thin moniliform prolongations (telopodes) extended from the cell body (Figs. 1 and 2). Telocytes often accompanied the cardiomyocytes and their telopodes ended up on cardiomyocytes. However, telocytes with their long prolongations interconnected cardiomyocytes and accelerated their synchronously beating (Figs. 3 and 4 and Movie S1).


Telocytes accompanying cardiomyocyte in primary culture: two- and three-dimensional culture environment.

Zhou J, Zhang Y, Wen X, Cao J, Li D, Lin Q, Wang H, Liu Z, Duan C, Wu K, Wang C - J. Cell. Mol. Med. (2010)

Telocytes in primary culture at day 7. (A) Typical telocytes with fusiform cell body and two long prolongations (telopodes). (B) Telocytes with two or three prolongations were different from cardiac cells. Bar = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373485&req=5

fig01: Telocytes in primary culture at day 7. (A) Typical telocytes with fusiform cell body and two long prolongations (telopodes). (B) Telocytes with two or three prolongations were different from cardiac cells. Bar = 50 μm.
Mentions: In primary culture of cardiomyocytes isolated from myocardial tissues, the typical telocytes were detected. They have a fusiform cell body and two long prolongations (Fig. 1A). The morphology of telocytes in vitro is different from cardiac cells. After 7 days of culture, typical morphological features of telocytes appeared under light microscope: a small oval-shaped cell body with extremely long and thin moniliform prolongations (telopodes) extended from the cell body (Figs. 1 and 2). Telocytes often accompanied the cardiomyocytes and their telopodes ended up on cardiomyocytes. However, telocytes with their long prolongations interconnected cardiomyocytes and accelerated their synchronously beating (Figs. 3 and 4 and Movie S1).

Bottom Line: Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium.The results showed that typical telocytes are distributed among cardiomyocytes, connecting them by long telopodes.Also, a significant number of vimentin+ telocytes were present within engineered heart tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Republic of China.

Show MeSH