Epigenetic inactivation of the hsa-miR-203 in haematological malignancies.
Bottom Line: In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033).In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies.In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs.
Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. email@example.comShow MeSH
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Mentions: Upon transfection of mature miR-203 mimic which led to overexpression mature miR-203 (Fig. 4A), JEKO-1 showed 20% reduction in cell proliferation as compared with the negative control at 24th hr by MTT assay (Fig. 4B), and 2-fold increase of dead cells as measured by trypan blue exclusion assay (Fig. 4C), thereby suggesting the tumour suppressor role of miR-203 in lymphoma cells.
Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. firstname.lastname@example.org