Limits...
Epigenetic inactivation of the hsa-miR-203 in haematological malignancies.

Chim CS, Wong KY, Leung CY, Chung LP, Hui PK, Chan SY, Yu L - J. Cell. Mol. Med. (2011)

Bottom Line: In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033).In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies.In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. jcschim@hku.hk

Show MeSH

Related in: MedlinePlus

Effect of miR-203 in lymphoma cells. JEKO-1 cells, homozygously methylated for hsa-miR-203, were transfected with mature miR-203 mimic oligo as compared with non-targeting precursor control. (A) Stem-loop RT-qPCR analysis of mature miR-203 expression at 24 hrs after transfection. (B) Cellular proliferation of lymphoma cells in response to overexpression of miR-203 was measured by MTT assay. (C) Dead cells were measured by trypan blue exclusion assay. Error bars represent standard deviation.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4373446&req=5

fig04: Effect of miR-203 in lymphoma cells. JEKO-1 cells, homozygously methylated for hsa-miR-203, were transfected with mature miR-203 mimic oligo as compared with non-targeting precursor control. (A) Stem-loop RT-qPCR analysis of mature miR-203 expression at 24 hrs after transfection. (B) Cellular proliferation of lymphoma cells in response to overexpression of miR-203 was measured by MTT assay. (C) Dead cells were measured by trypan blue exclusion assay. Error bars represent standard deviation.

Mentions: Upon transfection of mature miR-203 mimic which led to overexpression mature miR-203 (Fig. 4A), JEKO-1 showed 20% reduction in cell proliferation as compared with the negative control at 24th hr by MTT assay (Fig. 4B), and 2-fold increase of dead cells as measured by trypan blue exclusion assay (Fig. 4C), thereby suggesting the tumour suppressor role of miR-203 in lymphoma cells.


Epigenetic inactivation of the hsa-miR-203 in haematological malignancies.

Chim CS, Wong KY, Leung CY, Chung LP, Hui PK, Chan SY, Yu L - J. Cell. Mol. Med. (2011)

Effect of miR-203 in lymphoma cells. JEKO-1 cells, homozygously methylated for hsa-miR-203, were transfected with mature miR-203 mimic oligo as compared with non-targeting precursor control. (A) Stem-loop RT-qPCR analysis of mature miR-203 expression at 24 hrs after transfection. (B) Cellular proliferation of lymphoma cells in response to overexpression of miR-203 was measured by MTT assay. (C) Dead cells were measured by trypan blue exclusion assay. Error bars represent standard deviation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373446&req=5

fig04: Effect of miR-203 in lymphoma cells. JEKO-1 cells, homozygously methylated for hsa-miR-203, were transfected with mature miR-203 mimic oligo as compared with non-targeting precursor control. (A) Stem-loop RT-qPCR analysis of mature miR-203 expression at 24 hrs after transfection. (B) Cellular proliferation of lymphoma cells in response to overexpression of miR-203 was measured by MTT assay. (C) Dead cells were measured by trypan blue exclusion assay. Error bars represent standard deviation.
Mentions: Upon transfection of mature miR-203 mimic which led to overexpression mature miR-203 (Fig. 4A), JEKO-1 showed 20% reduction in cell proliferation as compared with the negative control at 24th hr by MTT assay (Fig. 4B), and 2-fold increase of dead cells as measured by trypan blue exclusion assay (Fig. 4C), thereby suggesting the tumour suppressor role of miR-203 in lymphoma cells.

Bottom Line: In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033).In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies.In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. jcschim@hku.hk

Show MeSH
Related in: MedlinePlus