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Epigenetic inactivation of the hsa-miR-203 in haematological malignancies.

Chim CS, Wong KY, Leung CY, Chung LP, Hui PK, Chan SY, Yu L - J. Cell. Mol. Med. (2011)

Bottom Line: In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033).In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies.In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. jcschim@hku.hk

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Effect of 5-AzadC treatment on JEKO-1 lymphoma cells. (A) M-/U-MSP analysis of hsa-miR-203 promoter methylation status and (B) stem-loop RT-qPCR analysis of the mature miR-203 expression. 5-AzadC treatment resulted in progressive demethylation of hsa-miR-203 promoter, and re-expression of the mature miR-203 in JEKO-1 cells.
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fig03: Effect of 5-AzadC treatment on JEKO-1 lymphoma cells. (A) M-/U-MSP analysis of hsa-miR-203 promoter methylation status and (B) stem-loop RT-qPCR analysis of the mature miR-203 expression. 5-AzadC treatment resulted in progressive demethylation of hsa-miR-203 promoter, and re-expression of the mature miR-203 in JEKO-1 cells.

Mentions: JEKO-1 cells were completely methylated for hsa-miR-203. Upon 5-AzadC demethylation treatment, hsa-miR-203 U-MSP signal emerged on day 3 (Fig. 3A), with re-expression of mature miR-203 as shown by Taqman stem-loop RT-qPCR (Fig. 3B).


Epigenetic inactivation of the hsa-miR-203 in haematological malignancies.

Chim CS, Wong KY, Leung CY, Chung LP, Hui PK, Chan SY, Yu L - J. Cell. Mol. Med. (2011)

Effect of 5-AzadC treatment on JEKO-1 lymphoma cells. (A) M-/U-MSP analysis of hsa-miR-203 promoter methylation status and (B) stem-loop RT-qPCR analysis of the mature miR-203 expression. 5-AzadC treatment resulted in progressive demethylation of hsa-miR-203 promoter, and re-expression of the mature miR-203 in JEKO-1 cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373446&req=5

fig03: Effect of 5-AzadC treatment on JEKO-1 lymphoma cells. (A) M-/U-MSP analysis of hsa-miR-203 promoter methylation status and (B) stem-loop RT-qPCR analysis of the mature miR-203 expression. 5-AzadC treatment resulted in progressive demethylation of hsa-miR-203 promoter, and re-expression of the mature miR-203 in JEKO-1 cells.
Mentions: JEKO-1 cells were completely methylated for hsa-miR-203. Upon 5-AzadC demethylation treatment, hsa-miR-203 U-MSP signal emerged on day 3 (Fig. 3A), with re-expression of mature miR-203 as shown by Taqman stem-loop RT-qPCR (Fig. 3B).

Bottom Line: In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033).In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies.In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. jcschim@hku.hk

Show MeSH
Related in: MedlinePlus