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Epigenetic inactivation of the hsa-miR-203 in haematological malignancies.

Chim CS, Wong KY, Leung CY, Chung LP, Hui PK, Chan SY, Yu L - J. Cell. Mol. Med. (2011)

Bottom Line: In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033).In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies.In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. jcschim@hku.hk

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Related in: MedlinePlus

Methylation of hsa-miR-203. (A) U-MSP showed that the methylated control [M] was totally methylated, and all eight normal controls (N1–N8) were unmethylated. In the M-MSP, the methylated control was positive (methylated) but all normal controls were negative (unmethylated). (B) For the cell lines, SU-DHL-6, SU-DHL-16, JEKO-1, MINO, HL-60 and SKNO-1 were completely methylated of hsa-miR-203.
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fig01: Methylation of hsa-miR-203. (A) U-MSP showed that the methylated control [M] was totally methylated, and all eight normal controls (N1–N8) were unmethylated. In the M-MSP, the methylated control was positive (methylated) but all normal controls were negative (unmethylated). (B) For the cell lines, SU-DHL-6, SU-DHL-16, JEKO-1, MINO, HL-60 and SKNO-1 were completely methylated of hsa-miR-203.

Mentions: None of the eight normal control marrows showed aberrant methylation of hsa-miR-203 by MSP (Fig. 1A). The positive and negative controls showed the expected MSP results (normal DNA: U-MSP+/M-MSP–; methylated DNA: U-MSP–/M-MSP+).


Epigenetic inactivation of the hsa-miR-203 in haematological malignancies.

Chim CS, Wong KY, Leung CY, Chung LP, Hui PK, Chan SY, Yu L - J. Cell. Mol. Med. (2011)

Methylation of hsa-miR-203. (A) U-MSP showed that the methylated control [M] was totally methylated, and all eight normal controls (N1–N8) were unmethylated. In the M-MSP, the methylated control was positive (methylated) but all normal controls were negative (unmethylated). (B) For the cell lines, SU-DHL-6, SU-DHL-16, JEKO-1, MINO, HL-60 and SKNO-1 were completely methylated of hsa-miR-203.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373446&req=5

fig01: Methylation of hsa-miR-203. (A) U-MSP showed that the methylated control [M] was totally methylated, and all eight normal controls (N1–N8) were unmethylated. In the M-MSP, the methylated control was positive (methylated) but all normal controls were negative (unmethylated). (B) For the cell lines, SU-DHL-6, SU-DHL-16, JEKO-1, MINO, HL-60 and SKNO-1 were completely methylated of hsa-miR-203.
Mentions: None of the eight normal control marrows showed aberrant methylation of hsa-miR-203 by MSP (Fig. 1A). The positive and negative controls showed the expected MSP results (normal DNA: U-MSP+/M-MSP–; methylated DNA: U-MSP–/M-MSP+).

Bottom Line: In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033).In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies.In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China. jcschim@hku.hk

Show MeSH
Related in: MedlinePlus