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Crucial role of HSP90 in the Akt-dependent promotion of angiogenic-like effect of glucose-regulated protein94 (Grp94)-IgG complexes.

Tramentozzi E, Tibaldi E, Brunati AM, Pagetta A, Finotti P - J. Cell. Mol. Med. (2011)

Bottom Line: CCT failed to inhibit any morphological alteration induced by Grp94-IgG on HUVECs, on its own displaying a paradoxical angiogenic-like activity.CTT appeared to enhance the angiogenic-like effect of Grp94-IgG by increasing the rate of secretion of both HSP90 and MMP-9.Results reveal a fundamental role of HSP90 in the PI3K/Akt pathway-mediated angiogenic-like effect of Grp94-IgG, also questioning the capacity of CTT to serve as an effective inhibitor of the angiogenic effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Largo E. Meneghetti, Padova, Italy.

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PU-H71 inhibitor completely reverses the morphological alteration induced by Grp94-IgG complexes on HUVECs. Cells (25 × 104) were cultured in duplicate wells (2 ml) with Grp94-IgG (10ng/ml) and PU-H71 (50 and 150 nM), both alone and together, and after 18 hrs incubation morphology was visualized at the optical microscope. Representative pictures of each condition are presented with original magnification of 10 × .
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fig09: PU-H71 inhibitor completely reverses the morphological alteration induced by Grp94-IgG complexes on HUVECs. Cells (25 × 104) were cultured in duplicate wells (2 ml) with Grp94-IgG (10ng/ml) and PU-H71 (50 and 150 nM), both alone and together, and after 18 hrs incubation morphology was visualized at the optical microscope. Representative pictures of each condition are presented with original magnification of 10 × .

Mentions: Optical microscopy inspection also revealed that PU-H71 was able to restore, in a concentration-dependent manner, the normal morphology of HUVECs, the tubule-like formations induced by Grp94-IgG being completely antagonized at 150 nM PU-H71 (Fig. 9).


Crucial role of HSP90 in the Akt-dependent promotion of angiogenic-like effect of glucose-regulated protein94 (Grp94)-IgG complexes.

Tramentozzi E, Tibaldi E, Brunati AM, Pagetta A, Finotti P - J. Cell. Mol. Med. (2011)

PU-H71 inhibitor completely reverses the morphological alteration induced by Grp94-IgG complexes on HUVECs. Cells (25 × 104) were cultured in duplicate wells (2 ml) with Grp94-IgG (10ng/ml) and PU-H71 (50 and 150 nM), both alone and together, and after 18 hrs incubation morphology was visualized at the optical microscope. Representative pictures of each condition are presented with original magnification of 10 × .
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373444&req=5

fig09: PU-H71 inhibitor completely reverses the morphological alteration induced by Grp94-IgG complexes on HUVECs. Cells (25 × 104) were cultured in duplicate wells (2 ml) with Grp94-IgG (10ng/ml) and PU-H71 (50 and 150 nM), both alone and together, and after 18 hrs incubation morphology was visualized at the optical microscope. Representative pictures of each condition are presented with original magnification of 10 × .
Mentions: Optical microscopy inspection also revealed that PU-H71 was able to restore, in a concentration-dependent manner, the normal morphology of HUVECs, the tubule-like formations induced by Grp94-IgG being completely antagonized at 150 nM PU-H71 (Fig. 9).

Bottom Line: CCT failed to inhibit any morphological alteration induced by Grp94-IgG on HUVECs, on its own displaying a paradoxical angiogenic-like activity.CTT appeared to enhance the angiogenic-like effect of Grp94-IgG by increasing the rate of secretion of both HSP90 and MMP-9.Results reveal a fundamental role of HSP90 in the PI3K/Akt pathway-mediated angiogenic-like effect of Grp94-IgG, also questioning the capacity of CTT to serve as an effective inhibitor of the angiogenic effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Largo E. Meneghetti, Padova, Italy.

Show MeSH
Related in: MedlinePlus