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Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

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Related in: MedlinePlus

Hypoxia increases the invasion capacity of hCMPCs, and stimulates the production of pro-angiogenic factors. (A) hCMPC spheroids, embedded in collagen, invade collagen to form sprouts. Representative pictures of hCMPC spheroid sprouting under normoxia and hypoxia are shown. (B) The number of sprouts per spheroid increased under hypoxic conditions, whereas the average length of a sprout decreased (C). (D) CM of hCMPCs positively influences the sprouting capacity of endothelial cells. The number of sprouts per spheroid increased when HUVEC spheroids are cultured in the presence of 1% O2-CM, the length of a sprout was not affected (E).
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fig05: Hypoxia increases the invasion capacity of hCMPCs, and stimulates the production of pro-angiogenic factors. (A) hCMPC spheroids, embedded in collagen, invade collagen to form sprouts. Representative pictures of hCMPC spheroid sprouting under normoxia and hypoxia are shown. (B) The number of sprouts per spheroid increased under hypoxic conditions, whereas the average length of a sprout decreased (C). (D) CM of hCMPCs positively influences the sprouting capacity of endothelial cells. The number of sprouts per spheroid increased when HUVEC spheroids are cultured in the presence of 1% O2-CM, the length of a sprout was not affected (E).

Mentions: To determine the effect of hypoxia on the invasiveness of hCMPCs, we embedded these cells as spheroids in a 3D collagen gel. hCMPCs successfully formed spheroids that were able to form sprouts (Fig. 5A). When performing this assay under hypoxic conditions, we observed a significant increase in the number of sprouts invading the collagen layer and a decrease in the average length of the hypoxic sprouts (Fig. 5A–C).


Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Hypoxia increases the invasion capacity of hCMPCs, and stimulates the production of pro-angiogenic factors. (A) hCMPC spheroids, embedded in collagen, invade collagen to form sprouts. Representative pictures of hCMPC spheroid sprouting under normoxia and hypoxia are shown. (B) The number of sprouts per spheroid increased under hypoxic conditions, whereas the average length of a sprout decreased (C). (D) CM of hCMPCs positively influences the sprouting capacity of endothelial cells. The number of sprouts per spheroid increased when HUVEC spheroids are cultured in the presence of 1% O2-CM, the length of a sprout was not affected (E).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373441&req=5

fig05: Hypoxia increases the invasion capacity of hCMPCs, and stimulates the production of pro-angiogenic factors. (A) hCMPC spheroids, embedded in collagen, invade collagen to form sprouts. Representative pictures of hCMPC spheroid sprouting under normoxia and hypoxia are shown. (B) The number of sprouts per spheroid increased under hypoxic conditions, whereas the average length of a sprout decreased (C). (D) CM of hCMPCs positively influences the sprouting capacity of endothelial cells. The number of sprouts per spheroid increased when HUVEC spheroids are cultured in the presence of 1% O2-CM, the length of a sprout was not affected (E).
Mentions: To determine the effect of hypoxia on the invasiveness of hCMPCs, we embedded these cells as spheroids in a 3D collagen gel. hCMPCs successfully formed spheroids that were able to form sprouts (Fig. 5A). When performing this assay under hypoxic conditions, we observed a significant increase in the number of sprouts invading the collagen layer and a decrease in the average length of the hypoxic sprouts (Fig. 5A–C).

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

Show MeSH
Related in: MedlinePlus