Low oxygen tension positively influences cardiomyocyte progenitor cell function.
Bottom Line: After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.In conclusion, short exposure to hypoxia increases migratory and invasive capacities of hCMPCs and prolonged exposure induces proliferation, an angiogenic secretion profile and dampens migration, likely controlled by TSP-2.
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.Show MeSH
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Mentions: To determine the effect of hypoxia on the invasiveness of hCMPCs, we embedded these cells as spheroids in a 3D collagen gel. hCMPCs successfully formed spheroids that were able to form sprouts (Fig. 5A). When performing this assay under hypoxic conditions, we observed a significant increase in the number of sprouts invading the collagen layer and a decrease in the average length of the hypoxic sprouts (Fig. 5A–C).
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.