Low oxygen tension positively influences cardiomyocyte progenitor cell function.
Bottom Line: After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.In conclusion, short exposure to hypoxia increases migratory and invasive capacities of hCMPCs and prolonged exposure induces proliferation, an angiogenic secretion profile and dampens migration, likely controlled by TSP-2.
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.Show MeSH
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Mentions: The ability of progenitor cells to move through (scar) tissue is important for cells to reach the area that requires repair. Therefore we analysed the migration and invasion capacity of hCMPCs under hypoxia. Using a 2D scratch assay, we observed increased motility of hypoxic hCMPCs, resulting in a closure of 86%, whereas under normoxic conditions 41% was closed (Fig. 4A and B).
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.