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Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

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Migration capacity of hCMPCs is enhanced by hypoxia. hCMPCs secrete factors under hypoxia that improve the migration. (A) Representative pictures of cell migration after 4 hrs in scratch assay; hCMPCs migrate faster under hypoxia than under normoxia. (B) Quantification of migration in (A). (C) 1% O2-CM induced more migration than 20% O2-CM when the scratch assay was performed in 20% O2. (D) A Boyden chamber assay shows increased migration of hCMPCs towards 1% O2-CM. The number of migrated cells was normalized to unconditioned medium. (E) Gene expression of remodelers of ECM after 1 day of culturing was not significant influenced by hypoxia, except for TSP-2 mRNA expression which was increased under hypoxia. β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.
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fig04: Migration capacity of hCMPCs is enhanced by hypoxia. hCMPCs secrete factors under hypoxia that improve the migration. (A) Representative pictures of cell migration after 4 hrs in scratch assay; hCMPCs migrate faster under hypoxia than under normoxia. (B) Quantification of migration in (A). (C) 1% O2-CM induced more migration than 20% O2-CM when the scratch assay was performed in 20% O2. (D) A Boyden chamber assay shows increased migration of hCMPCs towards 1% O2-CM. The number of migrated cells was normalized to unconditioned medium. (E) Gene expression of remodelers of ECM after 1 day of culturing was not significant influenced by hypoxia, except for TSP-2 mRNA expression which was increased under hypoxia. β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.

Mentions: The ability of progenitor cells to move through (scar) tissue is important for cells to reach the area that requires repair. Therefore we analysed the migration and invasion capacity of hCMPCs under hypoxia. Using a 2D scratch assay, we observed increased motility of hypoxic hCMPCs, resulting in a closure of 86%, whereas under normoxic conditions 41% was closed (Fig. 4A and B).


Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Migration capacity of hCMPCs is enhanced by hypoxia. hCMPCs secrete factors under hypoxia that improve the migration. (A) Representative pictures of cell migration after 4 hrs in scratch assay; hCMPCs migrate faster under hypoxia than under normoxia. (B) Quantification of migration in (A). (C) 1% O2-CM induced more migration than 20% O2-CM when the scratch assay was performed in 20% O2. (D) A Boyden chamber assay shows increased migration of hCMPCs towards 1% O2-CM. The number of migrated cells was normalized to unconditioned medium. (E) Gene expression of remodelers of ECM after 1 day of culturing was not significant influenced by hypoxia, except for TSP-2 mRNA expression which was increased under hypoxia. β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373441&req=5

fig04: Migration capacity of hCMPCs is enhanced by hypoxia. hCMPCs secrete factors under hypoxia that improve the migration. (A) Representative pictures of cell migration after 4 hrs in scratch assay; hCMPCs migrate faster under hypoxia than under normoxia. (B) Quantification of migration in (A). (C) 1% O2-CM induced more migration than 20% O2-CM when the scratch assay was performed in 20% O2. (D) A Boyden chamber assay shows increased migration of hCMPCs towards 1% O2-CM. The number of migrated cells was normalized to unconditioned medium. (E) Gene expression of remodelers of ECM after 1 day of culturing was not significant influenced by hypoxia, except for TSP-2 mRNA expression which was increased under hypoxia. β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.
Mentions: The ability of progenitor cells to move through (scar) tissue is important for cells to reach the area that requires repair. Therefore we analysed the migration and invasion capacity of hCMPCs under hypoxia. Using a 2D scratch assay, we observed increased motility of hypoxic hCMPCs, resulting in a closure of 86%, whereas under normoxic conditions 41% was closed (Fig. 4A and B).

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

Show MeSH
Related in: MedlinePlus