Low oxygen tension positively influences cardiomyocyte progenitor cell function.
Bottom Line: After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.In conclusion, short exposure to hypoxia increases migratory and invasive capacities of hCMPCs and prolonged exposure induces proliferation, an angiogenic secretion profile and dampens migration, likely controlled by TSP-2.
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.Show MeSH
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Mentions: hCMPCs are able to differentiate into both cardiac and vascular cells in vitro and in vivo [3, 22, 23] and provide a unique biological system to explore the effect of hypoxia on cardiac progenitor cell differentiation. After 9 days of culture in a hypoxic environment, hCMPCs expressed the early cardiac transcription factors Nkx2.5 and myocardin indicating a commitment to the cardiac lineage. However, the sarcomeric genes MLC-2V and α-cardiac actin, which are specific for striated cardiomyocytes, were not observed. This shows that 9 days of only hypoxia is not sufficient to differentiate hCMPCs into cardiomyocytes (Fig. 2A). Low oxygen was also not sufficient to steer their differentiation into endothelial cells; only increased Tie-2 mRNA expression levels were observed (Fig. 2B).
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.