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Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

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Hypoxia does not induce differentiation of hCMPCs. Nine days of hypoxic culture did not induce expression of cardiomyocyte specific genes, as shown by RT-qPCR (A). Analysis of endothelial specific genes showed that there is no differentiation of hCMPCs into vascular cells during this incubation period (B). β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.
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fig02: Hypoxia does not induce differentiation of hCMPCs. Nine days of hypoxic culture did not induce expression of cardiomyocyte specific genes, as shown by RT-qPCR (A). Analysis of endothelial specific genes showed that there is no differentiation of hCMPCs into vascular cells during this incubation period (B). β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.

Mentions: hCMPCs are able to differentiate into both cardiac and vascular cells in vitro and in vivo [3, 22, 23] and provide a unique biological system to explore the effect of hypoxia on cardiac progenitor cell differentiation. After 9 days of culture in a hypoxic environment, hCMPCs expressed the early cardiac transcription factors Nkx2.5 and myocardin indicating a commitment to the cardiac lineage. However, the sarcomeric genes MLC-2V and α-cardiac actin, which are specific for striated cardiomyocytes, were not observed. This shows that 9 days of only hypoxia is not sufficient to differentiate hCMPCs into cardiomyocytes (Fig. 2A). Low oxygen was also not sufficient to steer their differentiation into endothelial cells; only increased Tie-2 mRNA expression levels were observed (Fig. 2B).


Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Hypoxia does not induce differentiation of hCMPCs. Nine days of hypoxic culture did not induce expression of cardiomyocyte specific genes, as shown by RT-qPCR (A). Analysis of endothelial specific genes showed that there is no differentiation of hCMPCs into vascular cells during this incubation period (B). β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373441&req=5

fig02: Hypoxia does not induce differentiation of hCMPCs. Nine days of hypoxic culture did not induce expression of cardiomyocyte specific genes, as shown by RT-qPCR (A). Analysis of endothelial specific genes showed that there is no differentiation of hCMPCs into vascular cells during this incubation period (B). β-actin was measured as a housekeeping gene, and data were normalized to day 0 expression. *Significant difference between normoxia and hypoxia.
Mentions: hCMPCs are able to differentiate into both cardiac and vascular cells in vitro and in vivo [3, 22, 23] and provide a unique biological system to explore the effect of hypoxia on cardiac progenitor cell differentiation. After 9 days of culture in a hypoxic environment, hCMPCs expressed the early cardiac transcription factors Nkx2.5 and myocardin indicating a commitment to the cardiac lineage. However, the sarcomeric genes MLC-2V and α-cardiac actin, which are specific for striated cardiomyocytes, were not observed. This shows that 9 days of only hypoxia is not sufficient to differentiate hCMPCs into cardiomyocytes (Fig. 2A). Low oxygen was also not sufficient to steer their differentiation into endothelial cells; only increased Tie-2 mRNA expression levels were observed (Fig. 2B).

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

Show MeSH
Related in: MedlinePlus