Low oxygen tension positively influences cardiomyocyte progenitor cell function.
Bottom Line: After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.In conclusion, short exposure to hypoxia increases migratory and invasive capacities of hCMPCs and prolonged exposure induces proliferation, an angiogenic secretion profile and dampens migration, likely controlled by TSP-2.
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.Show MeSH
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Mentions: Because hypoxia has been shown to induce the proliferation of several progenitor cell types , we analysed the growth rate of hCMPCs. During the first days of culture there were no differences in the proliferation of hCMPCs cultured under normoxic or hypoxic conditions (data not shown). However from day 6 onwards, hypoxic hCMPCs exhibited a higher cell density as was shown by labelling the cell cultures with fluorescein di-acetate (Fig. 1A). This increase in cell number was further consolidated by measuring the overall cellular mitochondrial respiratory chain activity using an MTT assay. Moreover, cellular DNA synthesis, a measure of cell division, was investigated by BrdU incorporation. Both assays revealed a significantly increased proliferation under low oxygen culture conditions (Fig. 1B and C).
Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.