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Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

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Related in: MedlinePlus

Hypoxia increases the proliferation of hCMPCs. hCMPCs were cultured under normoxic and hypoxic conditions for the indicated time intervals. An increase in the number of cells under hypoxia was visualized by staining with fluorescein di-acetate (A). Viability and cell cycle progression was quantified by MTT (B) and BrdU (C), respectively. Both assays show an increased proliferation under hypoxia. Values were normalized to normoxia of the same time-point and one representative experiment of seven is shown. *Significant difference between normoxia and hypoxia at the same time-point.
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fig01: Hypoxia increases the proliferation of hCMPCs. hCMPCs were cultured under normoxic and hypoxic conditions for the indicated time intervals. An increase in the number of cells under hypoxia was visualized by staining with fluorescein di-acetate (A). Viability and cell cycle progression was quantified by MTT (B) and BrdU (C), respectively. Both assays show an increased proliferation under hypoxia. Values were normalized to normoxia of the same time-point and one representative experiment of seven is shown. *Significant difference between normoxia and hypoxia at the same time-point.

Mentions: Because hypoxia has been shown to induce the proliferation of several progenitor cell types [7], we analysed the growth rate of hCMPCs. During the first days of culture there were no differences in the proliferation of hCMPCs cultured under normoxic or hypoxic conditions (data not shown). However from day 6 onwards, hypoxic hCMPCs exhibited a higher cell density as was shown by labelling the cell cultures with fluorescein di-acetate (Fig. 1A). This increase in cell number was further consolidated by measuring the overall cellular mitochondrial respiratory chain activity using an MTT assay. Moreover, cellular DNA synthesis, a measure of cell division, was investigated by BrdU incorporation. Both assays revealed a significantly increased proliferation under low oxygen culture conditions (Fig. 1B and C).


Low oxygen tension positively influences cardiomyocyte progenitor cell function.

van Oorschot AA, Smits AM, Pardali E, Doevendans PA, Goumans MJ - J. Cell. Mol. Med. (2011)

Hypoxia increases the proliferation of hCMPCs. hCMPCs were cultured under normoxic and hypoxic conditions for the indicated time intervals. An increase in the number of cells under hypoxia was visualized by staining with fluorescein di-acetate (A). Viability and cell cycle progression was quantified by MTT (B) and BrdU (C), respectively. Both assays show an increased proliferation under hypoxia. Values were normalized to normoxia of the same time-point and one representative experiment of seven is shown. *Significant difference between normoxia and hypoxia at the same time-point.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373441&req=5

fig01: Hypoxia increases the proliferation of hCMPCs. hCMPCs were cultured under normoxic and hypoxic conditions for the indicated time intervals. An increase in the number of cells under hypoxia was visualized by staining with fluorescein di-acetate (A). Viability and cell cycle progression was quantified by MTT (B) and BrdU (C), respectively. Both assays show an increased proliferation under hypoxia. Values were normalized to normoxia of the same time-point and one representative experiment of seven is shown. *Significant difference between normoxia and hypoxia at the same time-point.
Mentions: Because hypoxia has been shown to induce the proliferation of several progenitor cell types [7], we analysed the growth rate of hCMPCs. During the first days of culture there were no differences in the proliferation of hCMPCs cultured under normoxic or hypoxic conditions (data not shown). However from day 6 onwards, hypoxic hCMPCs exhibited a higher cell density as was shown by labelling the cell cultures with fluorescein di-acetate (Fig. 1A). This increase in cell number was further consolidated by measuring the overall cellular mitochondrial respiratory chain activity using an MTT assay. Moreover, cellular DNA synthesis, a measure of cell division, was investigated by BrdU incorporation. Both assays revealed a significantly increased proliferation under low oxygen culture conditions (Fig. 1B and C).

Bottom Line: Previously we observed that cardiomyocyte progenitor cells (hCMPCs) isolated from the human heart differentiate spontaneously into cardiomyocytes and vascular cells when transplanted after myocardial infarction (MI) in the ischemic heart.After MI, deprivation of oxygen is the first major change in the cardiac environment.Knockdown of TSP-2 resulted in increased proliferation, migration and MMP activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Cell Biology and Center for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

Show MeSH
Related in: MedlinePlus