Cyclic ADP ribose is a novel regulator of intracellular Ca2+ oscillations in human bone marrow mesenchymal stem cells.
Bottom Line: However, cADPR had no effect on adipogenesis or osteogenesis in human MSCs.Our results indicate that cADPR is a novel regulator of Ca(2+) (i) oscillations in human MSCs.It permeates the cell membrane through the nucleoside transporters and increases Ca(2+) oscillation via activation of the TRPM2 channel, resulting in enhanced phosphorylation of ERK1/2 and, thereby, stimulation of human MSC proliferation.
Affiliation: Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.Show MeSH
Mentions: Human MSCs (passage 4) showed a fibroblast-like appearance at day 3 after sub-culturing and became confluence at day 8 (Fig. 1). Figure 1B and C display adipogenic and osteogenic differentiation in human MSCs at passage 5. The cells could be induced to differentiate efficiently into adipocytes (Fig. 1B) or osteocytes (Fig. 1C), which occurred, however, only after specific induction. RT-PCR (Fig. 1D) revealed the expression of lineage-specific genes after induction: PPAR-γ and fatty acid binding protein 4 for adipocytes, and runt related transcription factor 2 and osteocalcin for osteocytes [2, 31]. The PCR primers used for detection of these specific genes are shown in Table S1. These results indicate that human MSCs we studied here are maintained in the ‘stem’ state before induction.
Affiliation: Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.