Palmitoylethanolamide counteracts reactive astrogliosis induced by β-amyloid peptide.
Bottom Line: This effect was reduced by PPAR-α antagonist.Moreover, this ALIAmide, like Aβ, increased 2-AG levels.These results indicate that PEA exhibits anti-inflammatory properties able to counteract Aβ-induced astrogliosis, and suggest novel treatment for neuroinflammatory/ neurodegenerative processes.
Affiliation: Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.Show MeSH
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Mentions: Further experiments were aimed at investigating whether the increase in the production of inflammatory factors induced by Aβ was dependent on the activation of the MAPK pathway. Activation of the MAPKs, p38 MAPK and JNK, was measured by quantifying the level of phosphorylation of each kinase by Western blot 30 min. after the exposure to Aβ (Fig. 4). Treatment of astrocytes with Aβ resulted in an increase in the level of phosphorylation of the kinases, and it was accompanied with the activation of the nuclear factor NF-κB and AP-1, as shown by EMSA analysis (Fig. 5). PEA significantly inhibited MAPK phosphorylation and nuclear transcription factors, NF-κB and AP-1. This inhibition was attenuated by the PPAR-α antagonist, MK886, in a partly, although significant, manner.
Affiliation: Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.