Palmitoylethanolamide counteracts reactive astrogliosis induced by β-amyloid peptide.
Bottom Line: Experiments were carried out to investigate PEA's (10(-7) M) effects upon the expression and release of pro-inflammatory molecules in rat primary astrocytes activated by soluble Aβ(1-42) (1 μg/ml) as well as to identify mechanisms responsible for such actions.This effect was reduced by PPAR-α antagonist.Moreover, this ALIAmide, like Aβ, increased 2-AG levels.
Affiliation: Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.Show MeSH
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Mentions: Another set of experiments was aimed at assessing the effect of PEA on the production of inflammatory factors induced by Aβ. Treatment with Aβ for 24 hrs resulted in a significant increase in iNOS and COX-2 expression, as determined by Western blot and immunofluorescence analyses. Such protein increase paralleled nitric oxide, IL-1β, TNF-α and PGE2 up-release, as determined by Griess reaction and ELISA experiments. Also in this case PEA antagonized the enhancement of both expression and release of all pro-inflammatory molecules detected, and the blockade of PPAR-α with MK886 partly attenuated these effects (Figs 2 and 3).
Affiliation: Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.