Palmitoylethanolamide counteracts reactive astrogliosis induced by β-amyloid peptide.
Bottom Line: Experiments were carried out to investigate PEA's (10(-7) M) effects upon the expression and release of pro-inflammatory molecules in rat primary astrocytes activated by soluble Aβ(1-42) (1 μg/ml) as well as to identify mechanisms responsible for such actions.This effect was reduced by PPAR-α antagonist.Moreover, this ALIAmide, like Aβ, increased 2-AG levels.
Affiliation: Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.Show MeSH
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Mentions: In order to test the effect of PEA on Aβ-induced astrogliosis, the expression of GFAP and S100B, specific markers of astrocyte activity, was explored. Reactive astrocytes display hypertrophied cell bodies and thickened processes exhibiting GFAP-immunoreactivity. Using immunofluorescence, Western blot and RT-PCR analyses, we observed a marked increase in both transcription and expression of GFAP after Aβ challenge. This enhancement was significantly attenuated by PEA treatment. Moreover, the PPAR-α antagonist MK886 was able to partly blunt the PEA-induced attenuation of GFAP transcription and expression. Such effect indicated a significant, although not exclusive, involvement of the PPAR-α receptor in mediating PEA action (Fig. 1).
Affiliation: Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.