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Human red blood cell aging: correlative changes in surface charge and cell properties.

Huang YX, Wu ZJ, Mehrishi J, Huang BT, Chen XY, Zheng XJ, Liu WJ, Luo M - J. Cell. Mol. Med. (2011)

Bottom Line: Therefore, to remove the ambiguity and uncertainty, we carried out multiparameteric studies on Percoll fractions of blood of 38 volunteers (lightest-young-Y-RBCs, densest-old-O-RBCs, two middle fractions).We found that there were striking differences between the properties of Y-RBCs and O-RBCs.The ζ-potential of Y-RBCs decreased gradually with aging.Close correlation was found between the surface charge on an aging RBC and its structure and functions, from the cell morphology, the membrane deformability to the intracellular Hb structure and oxidation ability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Engineering, Ji Nan University, Guang Zhou, China. tyxhuang@jnu.edu.cn

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The flow cytometry on the size and density of O-RBCs (left) and Y-RBCs (right).
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fig04: The flow cytometry on the size and density of O-RBCs (left) and Y-RBCs (right).

Mentions: Old RBCs more often were found to be spherocytes, although echinocytes were also observed. The contact area (27.5 ± 2.16 μm2) and the major axes (6.16 ± 0.22 μm) of aged cells were significantly smaller (P < 0.05) than those for Y-RBCs (28.31 ± 2.52 μm2 and 6.81 ± 027 μm, respectively). In O-RBC suspensions, cell aggregation was also observed. The flow cytometry measurements on huge numbers of RBCs shown in Figure 4 also indicate that the O-RBCs fractionated by Percoll have a greater density but are smaller in size than Y-RBCs.


Human red blood cell aging: correlative changes in surface charge and cell properties.

Huang YX, Wu ZJ, Mehrishi J, Huang BT, Chen XY, Zheng XJ, Liu WJ, Luo M - J. Cell. Mol. Med. (2011)

The flow cytometry on the size and density of O-RBCs (left) and Y-RBCs (right).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373432&req=5

fig04: The flow cytometry on the size and density of O-RBCs (left) and Y-RBCs (right).
Mentions: Old RBCs more often were found to be spherocytes, although echinocytes were also observed. The contact area (27.5 ± 2.16 μm2) and the major axes (6.16 ± 0.22 μm) of aged cells were significantly smaller (P < 0.05) than those for Y-RBCs (28.31 ± 2.52 μm2 and 6.81 ± 027 μm, respectively). In O-RBC suspensions, cell aggregation was also observed. The flow cytometry measurements on huge numbers of RBCs shown in Figure 4 also indicate that the O-RBCs fractionated by Percoll have a greater density but are smaller in size than Y-RBCs.

Bottom Line: Therefore, to remove the ambiguity and uncertainty, we carried out multiparameteric studies on Percoll fractions of blood of 38 volunteers (lightest-young-Y-RBCs, densest-old-O-RBCs, two middle fractions).We found that there were striking differences between the properties of Y-RBCs and O-RBCs.The ζ-potential of Y-RBCs decreased gradually with aging.Close correlation was found between the surface charge on an aging RBC and its structure and functions, from the cell morphology, the membrane deformability to the intracellular Hb structure and oxidation ability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Engineering, Ji Nan University, Guang Zhou, China. tyxhuang@jnu.edu.cn

Show MeSH
Related in: MedlinePlus