Human red blood cell aging: correlative changes in surface charge and cell properties.
Bottom Line: Therefore, to remove the ambiguity and uncertainty, we carried out multiparameteric studies on Percoll fractions of blood of 38 volunteers (lightest-young-Y-RBCs, densest-old-O-RBCs, two middle fractions).We found that there were striking differences between the properties of Y-RBCs and O-RBCs.The ζ-potential of Y-RBCs decreased gradually with aging.Close correlation was found between the surface charge on an aging RBC and its structure and functions, from the cell morphology, the membrane deformability to the intracellular Hb structure and oxidation ability.
Affiliation: Institute of Biomedical Engineering, Ji Nan University, Guang Zhou, China. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: Old RBCs more often were found to be spherocytes, although echinocytes were also observed. The contact area (27.5 ± 2.16 μm2) and the major axes (6.16 ± 0.22 μm) of aged cells were significantly smaller (P < 0.05) than those for Y-RBCs (28.31 ± 2.52 μm2 and 6.81 ± 027 μm, respectively). In O-RBC suspensions, cell aggregation was also observed. The flow cytometry measurements on huge numbers of RBCs shown in Figure 4 also indicate that the O-RBCs fractionated by Percoll have a greater density but are smaller in size than Y-RBCs.
Affiliation: Institute of Biomedical Engineering, Ji Nan University, Guang Zhou, China. firstname.lastname@example.org