Intracerebral transplantation of foetal neural stem cells improves brain dysfunction induced by intracerebral haemorrhage stroke in mice.
Bottom Line: Our results demonstrated that foetal NSCs could differentiate into neural axons and dendrites and astrocytes in both in vitro and in vivo conditions, demonstrated by positive double or triple staining with Hoechst, neuronal specific nuclear protein, neurofilaments and glial fibrillary acidic protein.The foetal NSCs may also produce neurotrophic and/or neuroprotective factors during culture, because the culture medium alone could partially improve functional performance.Thus, our data suggest that the foetal NSCs may be one of the therapeutic candidates for ICH.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.Show MeSH
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Mentions: Hoechst-positive NSCs transplanted into ICH haemorrhage lesion were found to appear in the haemorrhage core (Fig. 6A), the border of the lesion (Fig. 6B and C) and the injection sites (Fig. 6D and E), 31 days after induction of ICH. Of those cells, transplanted foetal NSCs has positive staining with Hoechst alone (Fig. 7A and B), NeuN alone (Fig. 7C and D), GFAP alone (Fig. 7E and F) and both of Hoechst and NeuN or Hoechst and GFAP (Fig. 7G and H). The differentiated neurons had double staining of Hoechst and NeuN, whereas the astrocytes had Hoechst and GFAP. Some of differentiated neurons had neuron axon and dendrites with positive staining of Hoechst, NeurN and NF (Fig. 8). Those could be compared with eurons and nerve fibres of normal SD mouse brain (Fig. 9). The transplanted cells with NF, NeuN or both positive staining were mostly allocated in the peri-hematomal sites, where only some with GFAP positive staining appeared. Most of the Hoechst/GFAP double-positive cells were found along the border of haemorrhagic core.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.