Intracerebral transplantation of foetal neural stem cells improves brain dysfunction induced by intracerebral haemorrhage stroke in mice.
Bottom Line: Our results demonstrated that foetal NSCs could differentiate into neural axons and dendrites and astrocytes in both in vitro and in vivo conditions, demonstrated by positive double or triple staining with Hoechst, neuronal specific nuclear protein, neurofilaments and glial fibrillary acidic protein.The foetal NSCs may also produce neurotrophic and/or neuroprotective factors during culture, because the culture medium alone could partially improve functional performance.Thus, our data suggest that the foetal NSCs may be one of the therapeutic candidates for ICH.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.Show MeSH
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Mentions: No animals died of the operation and anaesthesia during the experiment. The limb-placing test was performed in animals receiving the intracerebral injection of foetal NSCs, culture medium alone or PBS 3, 10, 17, 24 and 31 days after the induction of ICH, as shown in Figure 5. The score of limb-placing test gradually decreased in ICH animals receiving intracerebral injection of PBS or culture medium alone by time. There was a significant difference in animals with the culture medium alone between scores on days 3 and 31 (P < 0.05). The animals transplanted with foetal NSCs showed significantly better performance on limb placement test from 3 weeks and on after the induction of ICH, as compared with those with culture medium alone or PBS (Fig. 5, P < 0.05 or 0.001, respectively). The scores of limb-placing test in animals with NSCs transplantation on both post-ICH 24 and 31 days were significantly lower than those on 3 days (P < 0.01). There was also a significance difference of scores in animals transplanted with NSCs between days 24 and 31 after ICH.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.