Intracerebral transplantation of foetal neural stem cells improves brain dysfunction induced by intracerebral haemorrhage stroke in mice.
Bottom Line: Our results demonstrated that foetal NSCs could differentiate into neural axons and dendrites and astrocytes in both in vitro and in vivo conditions, demonstrated by positive double or triple staining with Hoechst, neuronal specific nuclear protein, neurofilaments and glial fibrillary acidic protein.The foetal NSCs may also produce neurotrophic and/or neuroprotective factors during culture, because the culture medium alone could partially improve functional performance.Thus, our data suggest that the foetal NSCs may be one of the therapeutic candidates for ICH.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.Show MeSH
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Mentions: To evaluate and confirm the reproducibility and stability of ICH model, two animals were killed and the brains were harvested to check the formation and size of intracerebral haematoma daily for 5 days after the induction of ICH. The results showed consistent finding that the formation and size of intracerebral haematoma were clearly detectable at 6 hrs (Fig. 4A), became a clear mass on day 2 and reached the maximal 3 days after the injection of collagenase (Fig 4B). The haematoma was shaped with the clear edge, and became oval and regular with the mass effect. Behavioural test demonstrated that the dysfunction of neural system was detectable from 6 hrs and on after the introduction of ICH and became more progressive by the time. A number of limb placing tests became more obviously abnormal 2–3 days after the introduction of ICH on the right site, including the paralysis of the left (contralateral) forelimb, ipsilateral ‘circle-like’ walking, delayed or little contraction of the left hindlimb, and difficulty of passing through the narrow wooden barriers and muscular inability of the left hindlimb. Furthermore, the brain was examined using nuclear magnetic resonance imaging 3.0T (Signa HDx, GE Healthcare, Pittsburgh, PA, USA) at scanning of different sequences (AX FSE T2WI, AX FSE T1WI and AX GRE T2*WI), with the thickness layer of 1.6 mm. The normal brain showed the uniform of the density at AX FSE T2W scan (Fig. 4C), whereas ICH brain had the round lesion with low density on the right side (Fig. 4D) 3 days after the induction of ICH. At high resolution of GRE T2WI, the lesion became clearer (Fig. 4E), as compared with the FSE T1WI resolution (Fig. 4F).
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.