Intracerebral transplantation of foetal neural stem cells improves brain dysfunction induced by intracerebral haemorrhage stroke in mice.
Bottom Line: Our results demonstrated that foetal NSCs could differentiate into neural axons and dendrites and astrocytes in both in vitro and in vivo conditions, demonstrated by positive double or triple staining with Hoechst, neuronal specific nuclear protein, neurofilaments and glial fibrillary acidic protein.The foetal NSCs may also produce neurotrophic and/or neuroprotective factors during culture, because the culture medium alone could partially improve functional performance.Thus, our data suggest that the foetal NSCs may be one of the therapeutic candidates for ICH.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.Show MeSH
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Mentions: In order to avoid the potential influence of cellular contacts and space connections in cell differentiation, the single cell was isolated, seeded and cultured with the differentiated induction for 7 days. The cell adhered on the plate, formed neurites and differentiated into neurons and astrocytes, as shown in Figure 3. Cells with Hoechst 33342-positve nuclei showed positive to the staining of neuron-specific marker MAP-2 (Fig. 3A). The neurons formed peripheral process like extensions which became more obvious with the mono-staining of MAP-2 (Fig. 3B). The cell with clear neural axons and dendrites in the intermediate phase of differentiation from NSCs to neurons was also detected (Fig. 3C). NSCs-differentiated astrocytes were detected in microscopy with (Fig. 3D) and without GFAP staining (Fig. 3E). Some showed the matured shape of astrocytes with rich and strong GFAP staining (Fig. 3F). The cytoplasm of the astrocytes retracted towards the nucleus, forming a contracted cell body.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.