Intracerebral transplantation of foetal neural stem cells improves brain dysfunction induced by intracerebral haemorrhage stroke in mice.
Bottom Line: Our results demonstrated that foetal NSCs could differentiate into neural axons and dendrites and astrocytes in both in vitro and in vivo conditions, demonstrated by positive double or triple staining with Hoechst, neuronal specific nuclear protein, neurofilaments and glial fibrillary acidic protein.The foetal NSCs may also produce neurotrophic and/or neuroprotective factors during culture, because the culture medium alone could partially improve functional performance.Thus, our data suggest that the foetal NSCs may be one of the therapeutic candidates for ICH.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.Show MeSH
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Mentions: Mouse foetal NSCs were seeded as the separate cell on day 0 (Fig. 2A). A few cells then started to adhere on the plate and showed neurite, while some cells were still floated as single or a small mass of cells on day 3 (Fig. 2B). Most of cells grew to about 10â€“100 neurospheres floating as the round mass (Fig. 2C), of which the shapes were regulatory and oval with condensed cell contacts and smooth surface and became the matured neurosphere as seen at high magnification (Fig. 2D and E). The immunostaining of nestins as the marker of NSCs was performed 2 hrs after the neurosphere adhesion on the plate. Most of the neurospheres showed nestin-staining to be positive (Fig. 2F). Morphologies of NSCs changed and NSCs differentiated into neurons and astrocytes 7 days after the induction with DMEM/F12/10% foetal bovine serum.
Affiliation: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.