Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice.
Bottom Line: ROS was significantly decreased by inhibitors of NADPH oxidase, but not by inhibitors of other ROS production systems.Nox2 and Nox4 siRNA significantly attenuated TNF-α-induced ROS and upregulation of IL-1β and IL-6 in cardiomyocytes.Our study highlights a novel TNF-α-induced chronic ventricular remodelling mechanism mediated by sequential regulation of Nox2 and Nox4 subunits.
Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: There was a significant increase in ANP gene expression at all three time points. Mice injected with TNF-α 1, 7 and 28 days previously showed a 1.5-, 9- and 4-fold increase, respectively, compared to controls (Fig. 4C). These data were consistent with immunoblotting analysis of ANP, which revealed a significant increase in ANP protein levels in TNF-α injected mice at all three time points compared to controls (Fig. 4D). α-SMA expressions detected by immunohistochemistry showed that the expression was increased in mice injected with TNF-α 7 and 28 days previously (Fig. 5). However, the significant difference in α-SMA expression level was observed in the mice 28 days post-injection.
Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. email@example.com