Limits...
Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice.

Moe KT, Yin NO, Naylynn TM, Khairunnisa K, Wutyi MA, Gu Y, Atan MS, Wong MC, Koh TH, Wong P - J. Cell. Mol. Med. (2011)

Bottom Line: ROS was significantly decreased by inhibitors of NADPH oxidase, but not by inhibitors of other ROS production systems.Nox2 and Nox4 siRNA significantly attenuated TNF-α-induced ROS and upregulation of IL-1β and IL-6 in cardiomyocytes.Our study highlights a novel TNF-α-induced chronic ventricular remodelling mechanism mediated by sequential regulation of Nox2 and Nox4 subunits.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. moe.kyaw.thu@nhc.com.sg

Show MeSH

Related in: MedlinePlus

Increased α-SMA expression in the ventricular tissues of TNF-α-injected mice. α-SMA expression was examined by immunohistochemistry using mouse anti-α-SMA antibody. The images were analysed using Image Pro Plus software 6.0 and the values were presented with arbitrary units ± S.D. *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4373429&req=5

fig05: Increased α-SMA expression in the ventricular tissues of TNF-α-injected mice. α-SMA expression was examined by immunohistochemistry using mouse anti-α-SMA antibody. The images were analysed using Image Pro Plus software 6.0 and the values were presented with arbitrary units ± S.D. *P < 0.05.

Mentions: There was a significant increase in ANP gene expression at all three time points. Mice injected with TNF-α 1, 7 and 28 days previously showed a 1.5-, 9- and 4-fold increase, respectively, compared to controls (Fig. 4C). These data were consistent with immunoblotting analysis of ANP, which revealed a significant increase in ANP protein levels in TNF-α injected mice at all three time points compared to controls (Fig. 4D). α-SMA expressions detected by immunohistochemistry showed that the expression was increased in mice injected with TNF-α 7 and 28 days previously (Fig. 5). However, the significant difference in α-SMA expression level was observed in the mice 28 days post-injection.


Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice.

Moe KT, Yin NO, Naylynn TM, Khairunnisa K, Wutyi MA, Gu Y, Atan MS, Wong MC, Koh TH, Wong P - J. Cell. Mol. Med. (2011)

Increased α-SMA expression in the ventricular tissues of TNF-α-injected mice. α-SMA expression was examined by immunohistochemistry using mouse anti-α-SMA antibody. The images were analysed using Image Pro Plus software 6.0 and the values were presented with arbitrary units ± S.D. *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373429&req=5

fig05: Increased α-SMA expression in the ventricular tissues of TNF-α-injected mice. α-SMA expression was examined by immunohistochemistry using mouse anti-α-SMA antibody. The images were analysed using Image Pro Plus software 6.0 and the values were presented with arbitrary units ± S.D. *P < 0.05.
Mentions: There was a significant increase in ANP gene expression at all three time points. Mice injected with TNF-α 1, 7 and 28 days previously showed a 1.5-, 9- and 4-fold increase, respectively, compared to controls (Fig. 4C). These data were consistent with immunoblotting analysis of ANP, which revealed a significant increase in ANP protein levels in TNF-α injected mice at all three time points compared to controls (Fig. 4D). α-SMA expressions detected by immunohistochemistry showed that the expression was increased in mice injected with TNF-α 7 and 28 days previously (Fig. 5). However, the significant difference in α-SMA expression level was observed in the mice 28 days post-injection.

Bottom Line: ROS was significantly decreased by inhibitors of NADPH oxidase, but not by inhibitors of other ROS production systems.Nox2 and Nox4 siRNA significantly attenuated TNF-α-induced ROS and upregulation of IL-1β and IL-6 in cardiomyocytes.Our study highlights a novel TNF-α-induced chronic ventricular remodelling mechanism mediated by sequential regulation of Nox2 and Nox4 subunits.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. moe.kyaw.thu@nhc.com.sg

Show MeSH
Related in: MedlinePlus