Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice.
Bottom Line: ROS was significantly decreased by inhibitors of NADPH oxidase, but not by inhibitors of other ROS production systems.Nox2 and Nox4 siRNA significantly attenuated TNF-α-induced ROS and upregulation of IL-1β and IL-6 in cardiomyocytes.Our study highlights a novel TNF-α-induced chronic ventricular remodelling mechanism mediated by sequential regulation of Nox2 and Nox4 subunits.
Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: TNF-α injection into the mice resulted in hypertrophy of cardiomyocytes 7 and 28 days post-injection. The myocyte cross-sectional area showed a significant increase in mice injected with TNF-α 7 and 28 days previously compared to controls, while there was no significant change in myocyte area between day-1 treated and control mice (Fig. 4A). The heart and body weight ratio (HW/BW) also increased in mice injected with TNF-α 7 and 28 days previously (Fig. 4B). However, the significant difference was observed only mice 28 days post-injection.
Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. email@example.com