Nox2 and Nox4 mediate tumour necrosis factor-α-induced ventricular remodelling in mice.
Bottom Line: ROS was significantly decreased by inhibitors of NADPH oxidase, but not by inhibitors of other ROS production systems.Nox2 and Nox4 siRNA significantly attenuated TNF-α-induced ROS and upregulation of IL-1β and IL-6 in cardiomyocytes.Our study highlights a novel TNF-α-induced chronic ventricular remodelling mechanism mediated by sequential regulation of Nox2 and Nox4 subunits.
Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: Ventricular homogenates from both TNF-α-injected and control mice were used to determine Nox2 and Nox4 mRNA levels by real-time PCR and protein levels by immunoblotting. Analysis of Nox2 mRNA revealed a significant increase in mice injected with TNF-α 1 day previously (1.6-fold) compared to controls (Fig. 2A). However, no significant difference was observed between Nox2 mRNA levels of mice injected with TNF-α 7 and 28 days previously and control mice.
Affiliation: Research and Development Unit, National Heart Centre Singapore, Singapore. email@example.com