Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.
Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.
Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.Show MeSH
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Mentions: Given that accumulative evidence demonstrated that diabetes attenuates VEGF production and diminished levels of HIF1-α have been attributed to decreased VEGF in diabetic wound, expression of VEGF and HIF1-α in diabetic flap was evaluated in this study (Fig. 5A, D and E). Although no significant difference in the expression of VEGF was detectable between normal and diabetic skin, its expression was up-regulated in the skin flap at 24 hrs after elevation (Fig. 5A and D). Similar phenomena were also observed on the expression of HIF1-α (Fig. 5A and E). Moreover, expression of VEGF and HIF1-α in the diabetic flap was significantly down-regulated when compared to that in non-diabetic flap at 24-hr post-injury.
Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.