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Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

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ASCs augment HIF-1α and VEGF expression in the flap tissue of diabetic mice. HIF-1α and VEGF levels in ischaemic skin segments of non-diabetic and diabetic mice were measured 72 hrs after surgery. (A) Flaps from diabetic mice produced less VEGF and HIF-1α than non-diabetic ones. (B) VEGF protein expression was significantly augmented in ischaemic skin segments of diabetic mice in ASCs group. (C) HIF-1α protein expression was significantly augmented in ischaemic skin segments of STZ-diabetic mice in ASCs group. (D, E) Western blot quantitation of (A). n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus diabetic, respectively. (F, G) Western blot quantitation of (B) and (C), respectively. n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank; D: diabetic; ND: non-diabetic.
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fig05: ASCs augment HIF-1α and VEGF expression in the flap tissue of diabetic mice. HIF-1α and VEGF levels in ischaemic skin segments of non-diabetic and diabetic mice were measured 72 hrs after surgery. (A) Flaps from diabetic mice produced less VEGF and HIF-1α than non-diabetic ones. (B) VEGF protein expression was significantly augmented in ischaemic skin segments of diabetic mice in ASCs group. (C) HIF-1α protein expression was significantly augmented in ischaemic skin segments of STZ-diabetic mice in ASCs group. (D, E) Western blot quantitation of (A). n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus diabetic, respectively. (F, G) Western blot quantitation of (B) and (C), respectively. n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank; D: diabetic; ND: non-diabetic.

Mentions: Given that accumulative evidence demonstrated that diabetes attenuates VEGF production and diminished levels of HIF1-α have been attributed to decreased VEGF in diabetic wound, expression of VEGF and HIF1-α in diabetic flap was evaluated in this study (Fig. 5A, D and E). Although no significant difference in the expression of VEGF was detectable between normal and diabetic skin, its expression was up-regulated in the skin flap at 24 hrs after elevation (Fig. 5A and D). Similar phenomena were also observed on the expression of HIF1-α (Fig. 5A and E). Moreover, expression of VEGF and HIF1-α in the diabetic flap was significantly down-regulated when compared to that in non-diabetic flap at 24-hr post-injury.


Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

ASCs augment HIF-1α and VEGF expression in the flap tissue of diabetic mice. HIF-1α and VEGF levels in ischaemic skin segments of non-diabetic and diabetic mice were measured 72 hrs after surgery. (A) Flaps from diabetic mice produced less VEGF and HIF-1α than non-diabetic ones. (B) VEGF protein expression was significantly augmented in ischaemic skin segments of diabetic mice in ASCs group. (C) HIF-1α protein expression was significantly augmented in ischaemic skin segments of STZ-diabetic mice in ASCs group. (D, E) Western blot quantitation of (A). n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus diabetic, respectively. (F, G) Western blot quantitation of (B) and (C), respectively. n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank; D: diabetic; ND: non-diabetic.
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fig05: ASCs augment HIF-1α and VEGF expression in the flap tissue of diabetic mice. HIF-1α and VEGF levels in ischaemic skin segments of non-diabetic and diabetic mice were measured 72 hrs after surgery. (A) Flaps from diabetic mice produced less VEGF and HIF-1α than non-diabetic ones. (B) VEGF protein expression was significantly augmented in ischaemic skin segments of diabetic mice in ASCs group. (C) HIF-1α protein expression was significantly augmented in ischaemic skin segments of STZ-diabetic mice in ASCs group. (D, E) Western blot quantitation of (A). n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus diabetic, respectively. (F, G) Western blot quantitation of (B) and (C), respectively. n = 4, *P < 0.05 versus skin. n = 4, #P < 0.05 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank; D: diabetic; ND: non-diabetic.
Mentions: Given that accumulative evidence demonstrated that diabetes attenuates VEGF production and diminished levels of HIF1-α have been attributed to decreased VEGF in diabetic wound, expression of VEGF and HIF1-α in diabetic flap was evaluated in this study (Fig. 5A, D and E). Although no significant difference in the expression of VEGF was detectable between normal and diabetic skin, its expression was up-regulated in the skin flap at 24 hrs after elevation (Fig. 5A and D). Similar phenomena were also observed on the expression of HIF1-α (Fig. 5A and E). Moreover, expression of VEGF and HIF1-α in the diabetic flap was significantly down-regulated when compared to that in non-diabetic flap at 24-hr post-injury.

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

Show MeSH
Related in: MedlinePlus