Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.
Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.
Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.Show MeSH
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Mentions: Several previous studies reported that cellular migration in diabetic wounds was severely impaired. Hence, to study whether impaired cellular migration is responsible for the failure of ASCs in participating into blood vessel wall in diabetic flap, migration assay of ASCs cultured under HG and hypoxic circumstances was performed (Fig. 4A and B). In normoxia conditions, migration of ASCs grown in HG medium was similar to those grown in LG without significant difference (P > 0.05). However, exposure to hypoxia for 24 hrs resulted in a remarkable increase in migration of ASCs, either cultured at low or HG levels, compared to the respective ones at the normoxia condition. No significant difference was detected between the LG- and HG-treated cells under the same hypoxia condition. From the above results, it seems that migration of ASCs was not impaired when they were exposed to the HG and hypoxia in vitro, an environment mimicking what happened in the ischaemic skin flap in diabetic mice.
Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.