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Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

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Migration and differentiation of Dii-labelled ASCs in the flap of diabetic mice by post-operative day 7 (A–E) and day 14 (F–J). (A, F) Nuclei (blue) were stained with Hoechst. (B, G) Endothelial cells (green) were immunostained with an anti-CD31 antibody. (C, H) ASCs labelled by Dii (red) appeared in the flap tissue. (D) Merged images of A–C. (I) Merged image of F–H. (E, J) Localized magnification of D and I. One of the engrafted Dii-labelled ASCs was found incorporated into the blood vessel wall (yellow) indicated by a white arrow in E. Scale bars: 100 μm (A–I) and 25 μm (E, J).
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fig03: Migration and differentiation of Dii-labelled ASCs in the flap of diabetic mice by post-operative day 7 (A–E) and day 14 (F–J). (A, F) Nuclei (blue) were stained with Hoechst. (B, G) Endothelial cells (green) were immunostained with an anti-CD31 antibody. (C, H) ASCs labelled by Dii (red) appeared in the flap tissue. (D) Merged images of A–C. (I) Merged image of F–H. (E, J) Localized magnification of D and I. One of the engrafted Dii-labelled ASCs was found incorporated into the blood vessel wall (yellow) indicated by a white arrow in E. Scale bars: 100 μm (A–I) and 25 μm (E, J).

Mentions: To determine whether transplanted ASCs incorporated into vessels wall, by which angiogenesis was enhanced, we pre-labelled cells with fluorescent CM-Dii before transplantation. The distribution of labelled cells was observed on day 7 (Fig. 3). The survival of transplanted ASCs was demonstrated by the presence of labelled cells, most of which localized subcutaneously in a pattern of aggregation close to vasculature. However, little labelled cells could be detected in the wall of blood vessels.


Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

Migration and differentiation of Dii-labelled ASCs in the flap of diabetic mice by post-operative day 7 (A–E) and day 14 (F–J). (A, F) Nuclei (blue) were stained with Hoechst. (B, G) Endothelial cells (green) were immunostained with an anti-CD31 antibody. (C, H) ASCs labelled by Dii (red) appeared in the flap tissue. (D) Merged images of A–C. (I) Merged image of F–H. (E, J) Localized magnification of D and I. One of the engrafted Dii-labelled ASCs was found incorporated into the blood vessel wall (yellow) indicated by a white arrow in E. Scale bars: 100 μm (A–I) and 25 μm (E, J).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373426&req=5

fig03: Migration and differentiation of Dii-labelled ASCs in the flap of diabetic mice by post-operative day 7 (A–E) and day 14 (F–J). (A, F) Nuclei (blue) were stained with Hoechst. (B, G) Endothelial cells (green) were immunostained with an anti-CD31 antibody. (C, H) ASCs labelled by Dii (red) appeared in the flap tissue. (D) Merged images of A–C. (I) Merged image of F–H. (E, J) Localized magnification of D and I. One of the engrafted Dii-labelled ASCs was found incorporated into the blood vessel wall (yellow) indicated by a white arrow in E. Scale bars: 100 μm (A–I) and 25 μm (E, J).
Mentions: To determine whether transplanted ASCs incorporated into vessels wall, by which angiogenesis was enhanced, we pre-labelled cells with fluorescent CM-Dii before transplantation. The distribution of labelled cells was observed on day 7 (Fig. 3). The survival of transplanted ASCs was demonstrated by the presence of labelled cells, most of which localized subcutaneously in a pattern of aggregation close to vasculature. However, little labelled cells could be detected in the wall of blood vessels.

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

Show MeSH
Related in: MedlinePlus