Limits...
Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

Show MeSH

Related in: MedlinePlus

Microphotographs representing the comparative results of vascular density in the flaps of three groups. (A–C) Haematoxylin and eosin stains of the distal portion of flaps at 7 days post-operation of ASCs, medium- and blank-treated groups, showing increased neovascularization in the flap tissue of the ASCs group. (D–F) Frozen sections (8 μm) from the distal portion of random cutaneous flap tissue of three groups were stained with anti-CD31 antibody. The experiment was repeated three times, and one representative result is shown. (G) The number of blood vessels per high power field (HPF) was counted. n = 9, *P < 0.01 versus M and B groups, respectively. (H) The number of CD31-positive cells per HPF was counted. n = 9, *P < 0.01 versus M and B groups, respectively. Scale bars: 100 μm. A: ASCs-treated group; M: medium-treated group; B: blank.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4373426&req=5

fig02: Microphotographs representing the comparative results of vascular density in the flaps of three groups. (A–C) Haematoxylin and eosin stains of the distal portion of flaps at 7 days post-operation of ASCs, medium- and blank-treated groups, showing increased neovascularization in the flap tissue of the ASCs group. (D–F) Frozen sections (8 μm) from the distal portion of random cutaneous flap tissue of three groups were stained with anti-CD31 antibody. The experiment was repeated three times, and one representative result is shown. (G) The number of blood vessels per high power field (HPF) was counted. n = 9, *P < 0.01 versus M and B groups, respectively. (H) The number of CD31-positive cells per HPF was counted. n = 9, *P < 0.01 versus M and B groups, respectively. Scale bars: 100 μm. A: ASCs-treated group; M: medium-treated group; B: blank.

Mentions: As reported that ASCs could improve the survival of ischaemic skin flap through accelerating revascularization, we then investigated capillary densities in ischaemic skin flap of diabetic mice as shown in Figure 2. Histological observation of the flap tissue, which was obtained just adjacent to the necrotic boundary in each group, showed characterizations of normal skin with intact epidermis and dermis (Fig. 2A–C). However, samples in group A displayed an increased distribution of capillary vessels, which exhibited dilatant lumina as compared with vessels in groups B and C (Fig. 2A–C and G). To further investigate neovascularization in skin flaps, capillary densities at 7 days were assessed morphometrically by immunofluorescent staining for CD31 (Fig. 2D–F and H). Number of CD31+ cells was significantly higher in ASCs-treated group than that either in medium-treated group or in control flaps group (Fig. 2H).


Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

Microphotographs representing the comparative results of vascular density in the flaps of three groups. (A–C) Haematoxylin and eosin stains of the distal portion of flaps at 7 days post-operation of ASCs, medium- and blank-treated groups, showing increased neovascularization in the flap tissue of the ASCs group. (D–F) Frozen sections (8 μm) from the distal portion of random cutaneous flap tissue of three groups were stained with anti-CD31 antibody. The experiment was repeated three times, and one representative result is shown. (G) The number of blood vessels per high power field (HPF) was counted. n = 9, *P < 0.01 versus M and B groups, respectively. (H) The number of CD31-positive cells per HPF was counted. n = 9, *P < 0.01 versus M and B groups, respectively. Scale bars: 100 μm. A: ASCs-treated group; M: medium-treated group; B: blank.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373426&req=5

fig02: Microphotographs representing the comparative results of vascular density in the flaps of three groups. (A–C) Haematoxylin and eosin stains of the distal portion of flaps at 7 days post-operation of ASCs, medium- and blank-treated groups, showing increased neovascularization in the flap tissue of the ASCs group. (D–F) Frozen sections (8 μm) from the distal portion of random cutaneous flap tissue of three groups were stained with anti-CD31 antibody. The experiment was repeated three times, and one representative result is shown. (G) The number of blood vessels per high power field (HPF) was counted. n = 9, *P < 0.01 versus M and B groups, respectively. (H) The number of CD31-positive cells per HPF was counted. n = 9, *P < 0.01 versus M and B groups, respectively. Scale bars: 100 μm. A: ASCs-treated group; M: medium-treated group; B: blank.
Mentions: As reported that ASCs could improve the survival of ischaemic skin flap through accelerating revascularization, we then investigated capillary densities in ischaemic skin flap of diabetic mice as shown in Figure 2. Histological observation of the flap tissue, which was obtained just adjacent to the necrotic boundary in each group, showed characterizations of normal skin with intact epidermis and dermis (Fig. 2A–C). However, samples in group A displayed an increased distribution of capillary vessels, which exhibited dilatant lumina as compared with vessels in groups B and C (Fig. 2A–C and G). To further investigate neovascularization in skin flaps, capillary densities at 7 days were assessed morphometrically by immunofluorescent staining for CD31 (Fig. 2D–F and H). Number of CD31+ cells was significantly higher in ASCs-treated group than that either in medium-treated group or in control flaps group (Fig. 2H).

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

Show MeSH
Related in: MedlinePlus