Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.
Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.
Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.Show MeSH
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Mentions: As reported that ASCs could improve the survival of ischaemic skin flap through accelerating revascularization, we then investigated capillary densities in ischaemic skin flap of diabetic mice as shown in Figure 2. Histological observation of the flap tissue, which was obtained just adjacent to the necrotic boundary in each group, showed characterizations of normal skin with intact epidermis and dermis (Fig. 2A–C). However, samples in group A displayed an increased distribution of capillary vessels, which exhibited dilatant lumina as compared with vessels in groups B and C (Fig. 2A–C and G). To further investigate neovascularization in skin flaps, capillary densities at 7 days were assessed morphometrically by immunofluorescent staining for CD31 (Fig. 2D–F and H). Number of CD31+ cells was significantly higher in ASCs-treated group than that either in medium-treated group or in control flaps group (Fig. 2H).
Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.