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Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

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Related in: MedlinePlus

The survival area and microvascular blood flow of random-patterned skin flap in diabetic mice 7 days after flap elevation. (A) Survival area of ischaemic tissue assessed at 7 days after surgery. (B) Mean survival percentages of flaps among three groups. n = 8, P < 0.01 versus M and B groups, respectively. (C) Flap microvascular blood flow was measured using laser Doppler velocimetry. The measurements were performed 0.5 cm from the distal necrosis edge of the flap in nine mice. (D) Representative examples of microvascular blood flow measurement of three groups. Segments of 30 sec. are displayed. (E) Mean blood flow perfusion of flaps of three groups. n = 9, P < 0.01 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank.
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fig01: The survival area and microvascular blood flow of random-patterned skin flap in diabetic mice 7 days after flap elevation. (A) Survival area of ischaemic tissue assessed at 7 days after surgery. (B) Mean survival percentages of flaps among three groups. n = 8, P < 0.01 versus M and B groups, respectively. (C) Flap microvascular blood flow was measured using laser Doppler velocimetry. The measurements were performed 0.5 cm from the distal necrosis edge of the flap in nine mice. (D) Representative examples of microvascular blood flow measurement of three groups. Segments of 30 sec. are displayed. (E) Mean blood flow perfusion of flaps of three groups. n = 9, P < 0.01 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank.

Mentions: The surviving area in the flap was clearly distinguishable from necrotic one at day 7 post-operation in each group (Fig. 1A). By quantitative digital analysis, the mean proportion of flap viability in group A (ASCs transplantation group), group B (culture medium injection group) and group C (control group) was 83.2 ± 5.3%, 47.0 ± 10.5% and 43.7 ± 4.5%, respectively (Fig. 1B). The surviving area in group A was significantly higher than that either in group B (P < 0.01) or in group C (P < 0.01). However, no significant difference was found between group B and group C (P > 0.05).


Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α.

Gao W, Qiao X, Ma S, Cui L - J. Cell. Mol. Med. (2011)

The survival area and microvascular blood flow of random-patterned skin flap in diabetic mice 7 days after flap elevation. (A) Survival area of ischaemic tissue assessed at 7 days after surgery. (B) Mean survival percentages of flaps among three groups. n = 8, P < 0.01 versus M and B groups, respectively. (C) Flap microvascular blood flow was measured using laser Doppler velocimetry. The measurements were performed 0.5 cm from the distal necrosis edge of the flap in nine mice. (D) Representative examples of microvascular blood flow measurement of three groups. Segments of 30 sec. are displayed. (E) Mean blood flow perfusion of flaps of three groups. n = 9, P < 0.01 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373426&req=5

fig01: The survival area and microvascular blood flow of random-patterned skin flap in diabetic mice 7 days after flap elevation. (A) Survival area of ischaemic tissue assessed at 7 days after surgery. (B) Mean survival percentages of flaps among three groups. n = 8, P < 0.01 versus M and B groups, respectively. (C) Flap microvascular blood flow was measured using laser Doppler velocimetry. The measurements were performed 0.5 cm from the distal necrosis edge of the flap in nine mice. (D) Representative examples of microvascular blood flow measurement of three groups. Segments of 30 sec. are displayed. (E) Mean blood flow perfusion of flaps of three groups. n = 9, P < 0.01 versus M and B groups, respectively. A: ASCs-treated group; M: medium-treated group; B: blank.
Mentions: The surviving area in the flap was clearly distinguishable from necrotic one at day 7 post-operation in each group (Fig. 1A). By quantitative digital analysis, the mean proportion of flap viability in group A (ASCs transplantation group), group B (culture medium injection group) and group C (control group) was 83.2 ± 5.3%, 47.0 ± 10.5% and 43.7 ± 4.5%, respectively (Fig. 1B). The surviving area in group A was significantly higher than that either in group B (P < 0.01) or in group C (P < 0.01). However, no significant difference was found between group B and group C (P > 0.05).

Bottom Line: Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension.The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C.Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, 1st Teaching Hospital of Xinjiang Medical University, China.

Show MeSH
Related in: MedlinePlus