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Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers.

Sherman H, Frumin I, Gutman R, Chapnik N, Lorentz A, Meylan J, le Coutre J, Froy O - J. Cell. Mol. Med. (2011)

Bottom Line: However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR.We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF.Our results suggest that RF may share some benefits with those of CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.

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Circadian rhythms and average protein levels of inflammation markers in the liver and jejunum of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. Protein was analysed by Western blotting and quantified using actin as loading control. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 3 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).
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fig08: Circadian rhythms and average protein levels of inflammation markers in the liver and jejunum of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. Protein was analysed by Western blotting and quantified using actin as loading control. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 3 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).

Mentions: Tnfα did not oscillate in any of the tissues. Tnfα mRNA daily average levels significantly decreased in WAT (2.3-fold, P < 0.001, Student’s t-test), did not change in the jejunum, and slightly increased in the liver (1.3-fold, P < 0.05, Student’s t-test). Regardless of the mRNA levels, protein levels in both liver and jejunum showed an extensive reduction (3- and 2-fold, respectively, P < 0.01, Student’s t-test) (Fig. 8; Table S4).


Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers.

Sherman H, Frumin I, Gutman R, Chapnik N, Lorentz A, Meylan J, le Coutre J, Froy O - J. Cell. Mol. Med. (2011)

Circadian rhythms and average protein levels of inflammation markers in the liver and jejunum of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. Protein was analysed by Western blotting and quantified using actin as loading control. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 3 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373423&req=5

fig08: Circadian rhythms and average protein levels of inflammation markers in the liver and jejunum of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. Protein was analysed by Western blotting and quantified using actin as loading control. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 3 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).
Mentions: Tnfα did not oscillate in any of the tissues. Tnfα mRNA daily average levels significantly decreased in WAT (2.3-fold, P < 0.001, Student’s t-test), did not change in the jejunum, and slightly increased in the liver (1.3-fold, P < 0.05, Student’s t-test). Regardless of the mRNA levels, protein levels in both liver and jejunum showed an extensive reduction (3- and 2-fold, respectively, P < 0.01, Student’s t-test) (Fig. 8; Table S4).

Bottom Line: However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR.We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF.Our results suggest that RF may share some benefits with those of CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.

Show MeSH
Related in: MedlinePlus