Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers.
Bottom Line: However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR.We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF.Our results suggest that RF may share some benefits with those of CR.
Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.Show MeSH
Mentions: We next studied the effect of RF on several disease markers by assessing their phase, amplitude and overall expression levels around the circadian cycle. We measured PAI-1, a possible marker for thrombosis and proneness to heart attacks ; arginase, a possible marker for colorectal cancer and liver metastases [51–55]; growth arrest and DNA damage 45β (GADD45β), a possible marker for hepatocellular carcinoma ; α fetoprotein (AFP), a possible marker for hepatocellular carcinoma ; ALT, a possible marker for non-alcoholic fatty liver and obesity  and AST, a possible marker for hepatotoxicity . No robust oscillation at the mRNA level was observed in the expression of these disease markers except for liver Afp under RF (data not shown). ALT and ARGINASE protein levels did not oscillate as well (data not shown), suggesting that their genes are not controlled by the circadian clock. Although liver total Alt mRNA levels significantly increased (1.45-fold, P < 0.01, Student’s t-test), serum ALT and AST levels did not change (Fig. 6; Table S4). Similarly, no change was detected in average daily levels of Alt mRNA in the jejunum (Fig. 6; Table S4). However, average daily levels of jejunum Arginase, Gadd45β and Afp mRNA significantly decreased under RF (2-fold, 1.4-fold and 2-fold, respectively, P < 0.005, Student’s t-test) (Fig. 6; Table S4).
Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.