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Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers.

Sherman H, Frumin I, Gutman R, Chapnik N, Lorentz A, Meylan J, le Coutre J, Froy O - J. Cell. Mol. Med. (2011)

Bottom Line: However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR.We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF.Our results suggest that RF may share some benefits with those of CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.

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Circadian rhythms and average mRNA levels of metabolic markers in the liver, jejunum and WAT of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. mRNA was quantified by real-time PCR and is plotted as relative levels. Metabolic gene levels were normalized using Gapdh as the reference gene. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 4 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).
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fig03: Circadian rhythms and average mRNA levels of metabolic markers in the liver, jejunum and WAT of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. mRNA was quantified by real-time PCR and is plotted as relative levels. Metabolic gene levels were normalized using Gapdh as the reference gene. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 4 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).

Mentions: In the liver and jejunum, under AL, Ampk mRNA oscillation (P < 0.05, one-way anova) peaked during the light phase, the time of inactivity, whereas under RF, it peaked before food availability (P < 0.0001, one-way anova, Acro) (Fig. 3; Table S2). No oscillation could be detected in WAT. Liver AMPK protein oscillated under RF (P < 0.01, one-way anova) (Fig. 4; Table S3) with a phase delay compared to its mRNA (Fig. 3), as expected for the lag between transcription and translation. Interestingly, even the active phosphorylated form, pAMPK, exhibited circadian oscillation under both treatments (P < 0.01, one-way anova) (Fig. 4; Table S3), suggesting circadian control over the AMPK kinase. Analysis of daily average levels revealed no significant effect of RF on Ampk mRNA levels in liver, jejunum and WAT or on liver protein levels (Fig. 4; Table S4). However, pAMPK, the active AMPK form, significantly increased in the liver (1.4-fold, P < 0.05, Student’s t-test) and, thus, the ratio pAMPK/AMPK, attesting to the higher overall activation of AMPK under RF.


Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers.

Sherman H, Frumin I, Gutman R, Chapnik N, Lorentz A, Meylan J, le Coutre J, Froy O - J. Cell. Mol. Med. (2011)

Circadian rhythms and average mRNA levels of metabolic markers in the liver, jejunum and WAT of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. mRNA was quantified by real-time PCR and is plotted as relative levels. Metabolic gene levels were normalized using Gapdh as the reference gene. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 4 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).
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Related In: Results  -  Collection

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fig03: Circadian rhythms and average mRNA levels of metabolic markers in the liver, jejunum and WAT of AL- and RF-fed mice. Liver and jejunum were collected every 3 hrs around the circadian cycle from mice fed either AL (solid black line and columns) or RF (dashed grey line and grey columns). Food availability during RF is marked by the crosshatched box. mRNA was quantified by real-time PCR and is plotted as relative levels. Metabolic gene levels were normalized using Gapdh as the reference gene. For total daily levels, all time-points were averaged. The grey and black bars designate the subjective day and night, respectively. Values are means ± S.E., n = 4 for each time-point in each group. Asterisk denotes significant difference (P < 0.05).
Mentions: In the liver and jejunum, under AL, Ampk mRNA oscillation (P < 0.05, one-way anova) peaked during the light phase, the time of inactivity, whereas under RF, it peaked before food availability (P < 0.0001, one-way anova, Acro) (Fig. 3; Table S2). No oscillation could be detected in WAT. Liver AMPK protein oscillated under RF (P < 0.01, one-way anova) (Fig. 4; Table S3) with a phase delay compared to its mRNA (Fig. 3), as expected for the lag between transcription and translation. Interestingly, even the active phosphorylated form, pAMPK, exhibited circadian oscillation under both treatments (P < 0.01, one-way anova) (Fig. 4; Table S3), suggesting circadian control over the AMPK kinase. Analysis of daily average levels revealed no significant effect of RF on Ampk mRNA levels in liver, jejunum and WAT or on liver protein levels (Fig. 4; Table S4). However, pAMPK, the active AMPK form, significantly increased in the liver (1.4-fold, P < 0.05, Student’s t-test) and, thus, the ratio pAMPK/AMPK, attesting to the higher overall activation of AMPK under RF.

Bottom Line: However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR.We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF.Our results suggest that RF may share some benefits with those of CR.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.

Show MeSH
Related in: MedlinePlus