Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers.
Bottom Line: However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR.We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF.Our results suggest that RF may share some benefits with those of CR.
Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.Show MeSH
Mentions: In the liver and jejunum, under AL, Ampk mRNA oscillation (P < 0.05, one-way anova) peaked during the light phase, the time of inactivity, whereas under RF, it peaked before food availability (P < 0.0001, one-way anova, Acro) (Fig. 3; Table S2). No oscillation could be detected in WAT. Liver AMPK protein oscillated under RF (P < 0.01, one-way anova) (Fig. 4; Table S3) with a phase delay compared to its mRNA (Fig. 3), as expected for the lag between transcription and translation. Interestingly, even the active phosphorylated form, pAMPK, exhibited circadian oscillation under both treatments (P < 0.01, one-way anova) (Fig. 4; Table S3), suggesting circadian control over the AMPK kinase. Analysis of daily average levels revealed no significant effect of RF on Ampk mRNA levels in liver, jejunum and WAT or on liver protein levels (Fig. 4; Table S4). However, pAMPK, the active AMPK form, significantly increased in the liver (1.4-fold, P < 0.05, Student’s t-test) and, thus, the ratio pAMPK/AMPK, attesting to the higher overall activation of AMPK under RF.
Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.